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Effect of RNAi-mediated knockdown of unc-13 and Rbp on gene expression in motor neurons of Drosophila melanogaster third instar larva

ABSTRACT: Effect of RNAi-mediated knockdown of unc-13 and Rbp on gene expression in motor neurons of Drosophila melanogaster third instar larva

PROVIDER: PRJNA1054107 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27275800_1.fastq.gz	Fastqsanger.gz
	SRR27275800_2.fastq.gz	Fastqsanger.gz
	SRR27275801_1.fastq.gz	Fastqsanger.gz
	SRR27275801_2.fastq.gz	Fastqsanger.gz
	SRR27275802_1.fastq.gz	Fastqsanger.gz

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Effect of RNAi-mediated knockdown of unc-13 and Rbp on gene expression in motor neurons of Drosophila melanogaster third instar larva

Project description:Reliable neurotransmission is dependent on coordinating entry of calcium into the terminal boutons of a presynaptic neuron with the exocytosis of vesicles into the synaptic cleft. This function fulfilled by an assembly of proteins in a specialized region of the presynaptic plasma membrane called the active zone (AZ). Here we use the fruit fly neuromuscular junction as a model synapse to test the consequences of specific depletion of two key AZ proteins, unc-13 and RIM-binding protein (Rbp), by RNAi knockdown on the transcriptome of these motor neurons. We find that decreased expression of Rbp results in concomittant decrease of expression in unc-13 and that the sets of differentially expressed genes between the two knockdowns are highly similar. We show that these knockdowns result in increased expression of genes involved in neuropeptide signalling, while reducing expression of other genes encoding the components of the AZ and voltage-gated K+ channels. Taken together, our findings suggest that knockdown of AZ components results in gene expression changes that bias the presynaptic neuron towards increased firing to compensate for the loss of AZs.

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Transcriptomic analysis of third instar larva wing imaginal discs deregulating Cyclin G in Drosophila melanogaster

Project description:We report here the transcriptomic analysis of Drosophila melanogaster wing imaginal discs from third instar female larvae expressing Cyclin G deleted of the PEST domain (the 25 COOH-terminal amin-acids) under the control of the daugterless-Gal4 ubiquitous driver. The negative control was transgenic flies wearing only the daugterless-Gal4 driver.

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Genome-wide binding of Cyclin G in Drosophila melanogaster third instar larva wing imaginal discs.

Project description:We describe here the genome-wide binding sites of Cyclin G in Drosophila melanogaster wing imaginal discs from third instar female larvae. We used a transgenic line expressing a version Cyclin G deleted of the PEST domain (the 25 COOH-terminal amin-acids) and tagged by Myc under the control of the daugterless-Gal4 ubiquitous driver.

2017-07-01 | GSE99461 | GEO

Effect of RNAi-mediated knockdown of cac on gene expression in motor neurons of Drosophila melanogaster third instar larva

Project description:Entry of calcium into the terminal bouton of a neuron is critical step in action potential-dependent neurotransmitter release. At the Drosophila larval neuromuscular junction, the conversion of the presynaptic motor neuron’s electrical activity into a chemical signal is mediated by the voltage-gated calcium channel Cacophony (Cac). While RNAi knockdown of cac dramatically reduces the probability of evoked neurotransmitter release at the NMJ, locomotion remains largely unaffected (Hoagland et al., 2025). Here we investigate the mechanism of this compensation by sequencing the transcriptomes of Cac RNAi motor neurons. We find decreased expression of K+ channels as seen with the Rbp or unc-13 knockdown models of presynaptic dysfunction.

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Effect of RNAi-mediated knockdown of cac on gene expression in motor neurons of Drosophila melanogaster third instar larva

Project description:Effect of RNAi-mediated knockdown of cac on gene expression in motor neurons of Drosophila melanogaster third instar larva

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