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RNA-seq analysis of neuronal differentiation of KAT6A/B knockout mouse embryonic stem cells

ABSTRACT: RNA-seq analysis of neuronal differentiation of KAT6A/B knockout mouse embryonic stem cells

PROVIDER: PRJNA1054553 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27286129_1.fastq.gz	Fastqsanger.gz
	SRR27286129_2.fastq.gz	Fastqsanger.gz
	SRR27286130_1.fastq.gz	Fastqsanger.gz
	SRR27286130_2.fastq.gz	Fastqsanger.gz
	SRR27286131_1.fastq.gz	Fastqsanger.gz

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RNA-seq analysis of neuronal differentiation of KAT6A/B knockout mouse embryonic stem cells

Project description:Promoters of developmental genes in embryonic stem cells (ESCs) are marked by histone H3 lysine 4 trimethylation (H3K4me3) and H3K27me3 in an asymmetric nucleosomal conformation, with each sister histone H3 carrying one of the two marks. These so-called bivalent domains are thought to poise genes for timely activation upon differentiation. Here we show that asymmetric bivalent nucleosomes recruit repressive H3K27me3 binders but fail to enrich activating H3K4me3 binders, despite presence of H3K4me3, thereby promoting a poised state. Strikingly, the bivalent mark combination further promotes binding of chromatin proteins that are not recruited by each mark individually, including the histone acetyltransferase complex KAT6B (MORF). Knockout of KAT6B blocks neuronal differentiation, demonstrating that bivalency-specific readers are critical for proper ESC differentiation. These findings reveal how histone mark bivalency directly promotes establishment of a poised state at developmental genes, while highlighting how nucleosomal asymmetry is critical for histone mark readout and function.

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