Project description:The absence of Spib led to a notable upregulation of tuft cell-specific genes and transcripts typically associated with helminth infection characteristics
Project description:Genes significantly unregulated in Spib-deficient tuft cells included those associated with type 2 immune responses or induced by type 2 immune stimuli.
Project description:ChIP-seq data for the transcription factors (TFs) IRF4, PU.1 and SPIB from the cell lines OCI-LY3, OCI-LY10 and H929, and BATF from the cell lines OCI-Ly3 and OCI-Ly10. In addition ChIP-seq for the TFs IRF4, PU.1 and SPIB from the cell line OCI-LY3 following transfections of scramble/SPIB-siRNA. ChIP-seq data for the transcription factors (TFs) IRF4, PU.1 and SPIB from the cell lines OCI-LY3, OCI-LY10 and H929, and BATF from the cell lines OCI-Ly3 and OCI-Ly10. In addition ChIP-seq for the TFs IRF4, PU.1 and SPIB from the cell line OCI-LY3 following transfections of scramble/SPIB-siRNA.
Project description:SpiB, an Ets family transcription factor, is expressed exclusively in mature B cells, T-cell progenitors, and plasmacytoid dendritic cells. To understand the genes regulated by SpiB in epithelial cells, we overexpressed SpiB in lung cancer cell line A549 and examined the change of transcription profile using Agilent SurePrint G3 Human Gene Expression 8x60K v2 Microarray (Agilent Technologies).
Project description:To further characterize the influence of Mr BMT on the brains of ALSP mouse, we harvested brains from CSF1R WT/WT, naïve CSF1R WT/I792T and Mr BMT-treated CSF1R WT/I792T (deficient gene replaced by normal gene) mice for single-cell RNA-sequencing (scRNA-seq)