Atypical somatic small RNAs in Anopheles
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1056271 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR27334149.fastq.gz | Fastqsanger.gz | |||
| SRR27334150.fastq.gz | Fastqsanger.gz | |||
| SRR27334151.fastq.gz | Fastqsanger.gz | |||
| SRR27334152.fastq.gz | Fastqsanger.gz | |||
| SRR27334153.fastq.gz | Fastqsanger.gz |
Items per page: 5 1 - 5 of 12 |
Similar Datasets
Project description:Small RNAs of the Piwi-interacting RNA class (piRNA) play a key role in controlling the activity of transposable elements (TEs) in the animal germ line. In some arthropod species, including mosquitoes, the vectors of malaria and other pathogens, the piRNA pathway is active not only in the gonads, where it controls TE activity, but also in somatic tissues, where its targets and functions are less clear. It is thought that the piRNA pathway in somatic tissues is involved in the antiviral response and the modulation of TE activity. Here, we studied the features of small RNA production in head and thorax somatic tissues of Anopheles coluzzii focusing on the small RNAs processed from protein-coding gene mRNAs. We revealed tissue and sex specificity in the production of small RNAs derived from the genic transcripts. Genic small RNAs of 24-30 nt in length are atypical RNAs that lack the hallmarks of piRNAs. The majority of these short RNAs are derived from mitochondrial and nuclear genes involved in energy metabolism. We discuss the peculiarities of the piRNA biogenesis in Anopheles species which may result in the production of genic small RNAs in the somatic tissues.
2024-01-01 | GSE251974 | GEO
Project description:RNA silencing is a conserved mechanism in which small RNAs trigger various forms of sequence specific gene silencing by guiding Argonaute complexes to target RNAs via base-pairing. RNA silencing is thought to have evolved as a form of nucleic-acid-based immunity to inactivate viruses and transposable elements (TEs). Although the activity of TEs in animals has been thought to be largely restricted to the germline, recent studies have shown that they may also actively transpose in somatic cells, creating somatic mosaicism in animals3. In the Drosophila germline, piRNAs arise from repetitive intergenic elements including retrotransposons by a Dicer-independent pathway and function through the PIWI subfamily of Argonautes to ensure silencing of retrotransposons. Here we show that in Drosophila cultured S2 cells, Argonaute2 (AGO2), an AGO subfamily member of Argonautes, associates with endogenous small RNAs of 20-22 nucleotides in length, which we have collectively named esiRNAs (endogenous siRNAs). esiRNAs can be divided into two groups: one that mainly corresponds to a subset of retrotransposons, and the other that arises from stem-loop structures. esiRNAs are produced in a Dicer-2-dependent manner from distinctive genomic loci, are modified at their 3’ ends and can direct AGO2 to cleave target RNAs. Depletion of Dicer2 or AGO2 in S2 cells coincided with higher levels of retrotransposon transcripts. Together, our findings indicate that in Drosophila, different types of small RNAs and Argonautes are utilized to repress retrotransposons in germline and somatic cells. Keywords: AGO2, small RNA 454 sequencing - high throughput
2008-05-08 | GSE10277 | GEO
Project description:Comprehensive study of small RNA expression in Anopheles gambiae germinal tissues and larvae
| PRJEB7896 | ENA
Project description:Anopheles coluzzii small RNAseq
| PRJEB24723 | ENA
Project description:small RNA deep seq responses of Anopheles gambiae 3 days post arbovirus infection by artificial blood feeding
2018-06-28 | E-MTAB-6444 | biostudies-arrayexpress
Project description:To uncover the involvement of miRNAs and siRNAs in somatic embryogenesis of the perennial woody crop citrus, we carried out high-throughput (Illumina) sequencing (HTS) of sRNAs and RNA degradome tags in non-embryogenic and embryogenic tissue of Valencia sweet orange. A total of 191 stem-loop structures were identified, emanating 50 known and 45 novel miRNAs, 130 miniature inverted-repeat transposable elements (MITEs) derived small interfering RNAs (siRNAs) and 94 other siRNAs. Combining with the result of degradome sequencing, a total of 235 phased siRNAs (phasiRNAs), and 195 cleaved target genes were identified for miRNAs/siRNAs.
2015-01-01 | GSE53064 | GEO
Project description:Piwi-interacting small RNAs (piRNA) play a key role in controlling the activity of transposable elements (TEs) in the animal germline. In diverse arthropod species, including the pathogen vectors mosquitoes, the piRNA pathway is also active in nongonadal somatic tissues, where its targets and functions are less clear. Here, we studied the features of small RNA production in head and thorax tissues of an uninfected laboratory strain of Anopheles coluzzii focusing on the 24-32-nt-long RNAs. Small RNAs derived from repetitive elements constitute a minor fraction while most small RNAs process from long noncoding RNAs (lncRNAs) and protein-coding gene mRNAs. The majority of small RNAs derived from repetitive elements and lncRNAs exhibited typical piRNAs features. By contrast, majority of protein-coding gene-derived 24-32 nt small RNAs lack the hallmarks of piRNAs and have signatures of nontemplated 3' end tailing. Most of the atypical small RNAs exhibit female-biased expression and originate from mitochondrial and nuclear genes involved in energy metabolism. We also identified atypical genic small RNAs in Anopheles gambiae somatic tissues, which further validates the noncanonical mechanism of their production. We discuss a novel mechanism of small RNA production in mosquito somatic tissues and the possible functional significance of genic small RNAs.
| S-EPMC11834523 | biostudies-literature
Project description:Distribution of Small RNAs Along Transposable Elements in Vitis vinifera During Somatic Embryogenesis
| PRJNA633909 | ENA