Project description:Plant-derived nanovesicles (PDNVs) were isolated from Ginkgo biloba seed homogenate using differential ultracentrifugation (DUC) followed by density gradient ultracentrifugation (DGUC) with linear and non-linear iodixanol gradients. Nanoparticle tracking analysis (NTA) and cryo-transmission electron microscopy (cryo-TEM) characterized vesicle size, concentration, and morphology. Untargeted mass spectrometry profiled the protein content of distinct PDNV fractions. Ginkgo PDNVs formed a heterogeneous population, including single- and double-bilayer vesicles >50 nm. Proteomics revealed seed storage proteins (legumin, ginnacin) and membrane-associated ATPases, HSP90, catalase, PEPC, and eEF1A. Ginkgo seed-derived PDNVs exhibit promising vascular protective and anti-inflammatory properties, supporting their potential as safe, multifunctional agents for endothelial modulation.
Project description:Flavonoids are important medicinal active ingredients in Ginkgo biloba. However, the contribution of protein post-translational modification (PTM) in flavonoid biosynthesis has not been studied. Acetylation of lysine residues is a reversible protein PTM, which plays a key regulatory role in metabolism. To fully understand the molecular mechanism of flavonoid biosynthesis, the transcriptome, metabolome, proteome and lysine acetylome of different tissues of G. biloba were comprehensively analyzed.
Project description:Integrated multi-omics analysis provide insights into the importance of acetylation modification in flavonoid biosynthesis of Ginkgo biloba (PRJCA017404)
Project description:We conducted RNA-seq from the Ginkgo leaves after UV-B treatment,and constructed the molecular regulatory network of flavonoids synthesis under UV-B radiation in G. biloba.
