Project description:Centella asiatica extract is widely used in skincare and medicinal products due to its bioactive compounds, particularly triterpenoids, and their associated biological activities. Given the increasing demand for high-quality C. asiatica, it is worthwhile to investigate factors that can enhance the accumulation of these bioactive compounds and their bioactivities.
Project description:Centella asiatica is an important medicinal plant with a wide range of bioactivities associated with its secondary metabolites. Using two extraction procedures, metabolomic approaches were used to investigate changes in the metabolome of C. asiatica cells treated with exogenous MeJA. GC–MS and LC–MS platforms were employed for semi-targeted and untargeted analyses, respectively. Multivariate data analyses indicated concentration-dependent changes in the metabolite profiles, indicative of the cellular response to MeJA. Annotation of biomarkers correlated with the treatment indicate differential responses in flavonoid-, phenylpropanoid (cinnamates)- and terpenoid pathways and changes in fatty acid profiles. MeJA treatment triggered the accumulation of bicyclic sesquiterpenoids (aristolochene, deoxy-capsidiol, 15-hydroxysolavetivone, solavetivone, 3-hydroxylubimin) and a tricyclic sesquiterpenoid (phytuberin), indicating the stimulatory effect of MeJA on this branch of the terpenoid pathways. In contrast, flavonoids were mostly negatively correlated with the treatment. The presence of the sesquiterpenoids in MeJA-elicited cells and other tentatively identified metabolites (abscisic acid, fatty acids, phytosterols and metabolites of shikimate–phenylpropanoid pathways) indicates that the changes in the metabolome are associated with a defensive function in response to elicitation by MeJA, rather than just the amplification of existing terpene pathways. These results provide a detailed and comprehensive picture of metabolic changes occurring in C. asiatica cells in response to MeJA elicitation and contribute to the understanding of flexible and controllable aspects of metabolic manipulation.
