Project description:The roots of halophytes such as mangroves provide the first line of defense against the constant salt stress they experience. Such adaptation should include major reprogramming of the gene expression profiles. Using RNA-sequencing approach we identified 101,446 ‘all-unigenes’ from the seedling roots of the mangrove tree Avicennia officinalis. From the data 6618 genes were identified to be differentially regulated by salt when two-month-old greenhouse-grown seedlings without prior exposure to sea water were subjected to 24 h of 500 mM NaCl treatment. About 1,404 genes were significantly up-regulated, while 5214 genes were down-regulated. Based on Gene Ontology analysis, they could be classified under various categories, including metabolic processes, stress and defense response, signal transduction, transcription-related and transporters. Our analysis provides the baseline information towards understanding salt balance in mangroves and hence mechanism of salt tolerance in plants.
Project description:In the family Fagaceae, fertilization is delayed by several weeks to more than one year after pollination, leading to one- or two-year fruiting species depending on whether fruiting occurs in the same or the next year of flowering. Although delayed fertilization was recorded over a century ago, underlying mechanisms remain to be explored. To uncover the key genes associated with delayed fertilization, we obtain and analyze the comparative molecular phenology data over two years in one-year (Quercus glauca) and two-year fruiting species (Lithocarpus edulis).
Project description:To understand the molecular mechanisms of Suaeda salsa under salt stress, RNA-seq analysis was used to identify genes expressed in Suaeda salsa during salt stress response.
