Project description:Enhanced ammonia removal in tidal flow constructed wetland by incorporating steel slag: Performance, microbial community, and heavy metal release
| PRJNA717997 | ENA
Project description:16S rRNA and comamoA of secondary effluent constructed wetland
Project description:To unravel complex dynamics of environmental disturbance and microbial metabolic activities, we set up laboratory microcosms to investigate the effects of SO42- and O2 alone or in combination on microbial activities and interactions, as well as the resulting fate of carbon within wetland soil. We used proteogenomics to characterize the biochemical and physiological responses of microbial communities to individual perturbations and their combined effects. Stoichiometric models were employed to deconvolute carbon exchanges among the main functional guilds. These findings can contribute to the development of mechanistic models for predicting greenhouse gas emissions from wetland ecosystems under various climate change scenarios.
Project description:<p>Background and aims: Phosphate slag, a by-product of phosphorus and steel production, is a potential soil amendment that improves nutrient availability and redox stability. However, how it reshapes the rhizosphere microbiome and metabolism to promote tobacco (Nicotiana tabacum L.) root development remains unclear. This study aimed to elucidate the mechanisms by which phosphate slag reprograms rhizosphere microbial and metabolic networks, focusing on glutathione-centered redox regulation and carbon flux balance.</p><p>Methods: Metagenomic sequencing and untargeted metabolomics were integrated with soil and plant trait analyses in two tobacco-growing regions of Yunnan, China-Jiuxiang and Gengjiaying. Three treatments were tested: control (C), phosphate slag (P), and phosphate slag plus microbial conditioner (PM).</p><p>Results: Phosphate slag markedly reprogrammed the rhizosphere microbiome and metabolome, enriching taxa and pathways related to glutathione and ascorbate-aldarate metabolism, which enhanced antioxidant buffering and redox balance. Activation of the pentose phosphate pathway (PPP) supplied NADPH for glutathione recycling but reduced TCA flux and ATP synthesis, producing thicker but shorter roots. Increased siderophore and amino acid metabolism indicated improved nutrient mobilization. Multi-omics integration revealed coordinated microbe–metabolite interactions-microbes linked to potassium traits (TP, K₂O) and metabolites to sugar and alkaloid pools (TS, RS, TPA). Validation in Gengjiaying confirmed the reproducibility of these responses.</p><p>Conclusion: Phosphate slag enhances tobacco root growth by strengthening glutathione-mediated redox regulation and nutrient cycling. Yet, excessive PPP activation or microbial competition may limit energy supply. Balancing carbon and nutrient fluxes could optimize slag’s agronomic benefits and promote sustainable soil management.</p>
2026-01-16 | MTBLS13273 | MetaboLights
Project description:Profiling of Viral Communities in Steel Slag Leachate