Loropetalum chinense var. rubrum
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PROVIDER: PRJNA1058453 | ENA |
REPOSITORIES: ENA
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Project description:Loropetalum chinense var. rubrum genome
| PRJNA1058446 | ENA
Project description:<p><strong>BACKGROUND:</strong> <em>Loropetalum chinense </em>var.<em> rubrum</em> (<em>L. chinense</em> var. <em>rubrum</em>) is a precious, native, coloured-leaf ornamental plant in the Hunan Province. We found a <em>L. chinense</em> var. <em>rubrum</em> tree with 3 different leaf colours: GL (green leaf), ML (mosaic leaf) and PL (purple leaf). The mechanism of leaf coloration in this plant is still unclear. The aim of this study was to identify the metabolites and genes involved in determining the colour composition of <em>L. chinense</em> var. <em>rubrum</em> leaves, using phenotypic/anatomic observations, pigment content detection, and comparative metabolomics and transcriptomics.</p><p><strong>RESULTS:</strong> We observed that the mesophyll cells in PL were purple, those in GL were green and those in ML were a mix of purple-green. The contents of chlorophyll a, b, carotenoids and total chlorophyll in PL and ML were significantly lower than those in GL. While the anthocyanin content in PL and ML was significantly higher than that in GL. The metabolomics results showed that the differences in content of cyanidin 3-O-glucoside, delphinidin 3-O-glucoside, cyanidin 3,5-O-diglucoside, pelargonin and petunidin 3,5-diglucoside in ML, GL and PL were significant. Considering that the trend of anthocyanin content change was consistent with the leaf colour difference, we speculated that these compounds might influence the colour of <em>L. chinense</em> var. <em>rubrum</em> leaves. Finally, using transcriptomics, we identified 9 differentially expressed structural genes (one <em>ANR</em> (<em>ANR1217</em>); four <em>CYP75A</em> (<em>CYP75A1815</em>, <em>CYP75A2846</em>, <em>CYP75A2909</em> and <em>CYP75A1716</em>); four UFGTs (<em>UFGT1876</em>, <em>UFGT1649</em>, <em>UFGT1839</em> and <em>UFGT3273</em>) and nine transcription factors (two <em>MYBs</em> (<em>MYB1057</em> and <em>MYB1211</em>), one <em>MADS-box</em> (<em>MADS1235</em>), two <em>AP2-likes</em> (<em>AP2-like1779</em> and <em>AP2-like2234</em>), one <em>bZIP</em> (<em>bZIP3720</em>), two <em>WD40s</em> (<em>WD2173</em> and <em>WD1867</em>) and one <em>bHLH</em> (<em>bHLH1631</em>) that might be related to flavonoid biosynthesis that impacted the appearance of colour in <em>L. chinense</em> var. <em>rubrum</em> leaves.</p><p><strong>CONCLUSIONS:</strong> This study revealed potential molecular mechanisms associated with leaf coloration in <em>L. chinense</em> var. <em>rubrum</em> by analysing differential metabolites and genes related to the anthocyanin biosynthesis pathway. It also provides a reference for research on leaf colour variation in other ornamental plants.</p>
2023-01-30 | MTBLS5662 | MetaboLights
Project description:Loropetalum chinense var. rubrum Yieh (L. chinense) is an evergreen shrub or small tree of Hamamelidaceae. In this study, the chloroplast genome sequence of L. chinense is 159,451 bp in length, consisting of a large single-copy region with 88,166 bp (LSC), a small single-copy region with 18,773 bp (SSC), and two inverted repeat regions with 26,256 bp (IRs). The GC content in the chloroplast genome of L. chinense is 38.0%. The chloroplast genome of L. chinense contained 125 genes, including 84 protein-coding genes, 37 tRNA genes, and 4 rRNA genes. The phylogenetic tree showed that L. chinense was closely related to L. subcordatum.
| S-EPMC8667894 | biostudies-literature
Project description:Sargassum hemiphyllum var. chinense transcriptome data (PRJCA046586)
| PRJDB39457 | ENA
Project description:BackgroundLoropetalum chinense var. rubrum (L. chinense var. rubrum) is a precious, coloured-leaf native ornamental plant in the Hunan Province. We found an L. chinense var. rubrum tree with three different leaf colours: GL (green leaf), ML (mosaic leaf), and PL (purple leaf). The mechanism of leaf coloration in this plant is still unclear. Therefore, this study aimed to identify the metabolites and genes involved in determining the colour composition of L. chinense var. rubrum leaves, using phenotypic/anatomic observations, pigment content detection, and comparative metabolomics and transcriptomics.ResultsWe observed that the mesophyll cells in PL were purple, while those in GL were green and those in ML were a mix of purple-green. The contents of chlorophyll a, b, carotenoids, and total chlorophyll in PL and ML were significantly lower than those in GL. While the anthocyanin content in PL and ML was significantly higher than that in GL. The metabolomics results showed the differences in the content of cyanidin 3-O-glucoside, delphinidin 3-O-glucoside, cyanidin 3,5-O-diglucoside, pelargonidin, and petunidin 3,5-diglucoside in ML, GL, and PL were significant. Considering that the change trend of anthocyanin content change was consistent with the leaf colour difference, we speculated that these compounds might influence the colour of L. chinense var. rubrum leaves. Using transcriptomics, we finally identified nine differentially expressed structural genes (one ANR (ANR1217); four CYP75As (CYP75A1815, CYP75A2846, CYP75A2909, and CYP75A1716); four UFGTs (UFGT1876, UFGT1649, UFGT1839, and UFGT3273) and nine transcription factors (two MYBs (MYB1057 and MYB1211), one MADS-box (MADS1235), two AP2-likes (AP2-like1779 and AP2-like2234), one bZIP (bZIP3720), two WD40s (WD2173 and WD1867) and one bHLH (bHLH1631) that might be related to flavonoid biosynthesis and then impacted the appearance of colour in L. chinense var. rubrum leaves.ConclusionThis study revealed potential molecular mechanisms associated with leaf coloration in L. chinense var. rubrum by analyzing differential metabolites and genes related to the anthocyanin biosynthesis pathway. It also provided a reference for research on leaf colour variation in other ornamental plants.
| S-EPMC9993627 | biostudies-literature
Project description:"Pruning" is a simple and efficient way to control the flowering period, but it is rarely used in perennial woody ornamental plants. In this paper, Loropetalum chinense var. rubrum was pruned in different degrees, and the relationship between pruning intensity and flowering number, and flowering time and chlorophyll fluorescence parameters were compared. After statistics, it was found that pruning could advance blossoms of L. chinense var. rubrum; also, light and heavy cutting could both obtain a larger number of flowers. In addition, through correlation analysis, it was found that during the flowering period, the Rfd parameter of the unpruned treatment had a very significant positive correlation with the number of flowers FN, which was 0.81. In other pruning treatment groups, Rfd and FN also presented a certain positive correlation, indicating that the Rfd parameter can be used to predict the number of flowers during the flowering process of L. chinense var. rubrum. The research results provided a new idea for the regulation of the flowering period of L. chinense var. rubrum and other woody ornamental plants and laid the foundation for the diversified application of L. chinense var. rubrum.
| S-EPMC9104088 | biostudies-literature
Project description:IntroductionLoropetalum chinense var. rubrum blooms 2-3 times a year, among which the autumn flowering period has great potential for exploitation, but the number of flowers in the autumn flowering period is much smaller than that in the spring flowering period.MethodsUsing 'Hei Zhenzhu' and 'Xiangnong Xiangyun' as experimental materials, the winter growth environment of L. chinense var. rubrum in Changsha, Hunan Province was simulated by setting a low temperature of 6-10°C in an artificial climate chamber to investigate the effect of winter low temperature on the flowering traits and related gene expression of L. chinense var. rubrum.ResultsThe results showed that after 45 days of low temperature culture and a subsequent period of 25°C greenhouse culture, flower buds and flowers started to appear on days 24 and 33 of 25°C greenhouse culture for 'Hei Zhenzhu', and flower buds and flowers started to appear on days 21 and 33 of 25°C greenhouse culture for 'Xiangnong Xiangyun'. The absolute growth rate of buds showed a 'Up-Down' pattern during the 7-28 days of low temperature culture; the chlorophyll fluorescence decay rate (Rfd) of both materials showed a 'Down-Up-Down' pattern during this period. The non-photochemical quenching coefficient (NPQ) showed the same trend as Rfd, and the photochemical quenching coefficient (QP) fluctuated above and below 0.05. The expression of AP1 and FT similar genes of L. chinense var. rubrum gradually increased after the beginning of low temperature culture, reaching the highest expression on day 14 and day 28, respectively, and the expression of both in the experimental group was higher than that in the control group. The expressions of FLC, SVP and TFL1 similar genes all decreased gradually with low temperature culture, among which the expressions of FLC similar genes and TFL1 similar genes in the experimental group were extremely significantly lower than those in the control group; in the experimental group, the expressions of GA3 similar genes were all extremely significantly higher than those in the control group, and the expressions all increased with the increase of low temperature culture time.DiscussionWe found that the high expression of gibberellin genes may play an important role in the process of low temperature promotion of L. chinense var. rubrum flowering, and in the future, it may be possible to regulate L. chinense var. rubrum flowering by simply spraying exogenous gibberellin instead of the promotion effect of low temperature.
| S-EPMC9705732 | biostudies-literature