Similar Datasets
Project description:Microbiome DNA from the adhering fraction of a sheep rumen. The RSTs were generated using an improved version of SARST (referred to as iSARST) from the microbiome DNA extracted from the adhering fraction of the rumen content taken from a sheep. The iSARST method is going to be submitted to Nature Biotechnology for publication. Keywords: other
2004-10-15 | E-GEOD-1842 | biostudies-arrayexpress
Project description:The gut microbiome is a malleable microbial community that can remodel in response to various factors, including diet, and contribute to the development of several chronic diseases, including atherosclerosis. We devised an in vitro screening protocol of the mouse gut microbiome to discover molecules that can selectively modify bacterial growth. This approach was used to identify cyclic D,L-α-peptides that remodeled the Western diet (WD) gut microbiome toward the low-fat-diet microbiome state. Daily oral administration of the peptides in WD-fed LDLr-/- mice reduced plasma total cholesterol levels and atherosclerotic plaques. Depletion of the microbiome with antibiotics abrogated these effects. Peptide treatment reprogrammed the microbiome transcriptome, suppressed the production of pro-inflammatory cytokines (including interleukin-6, tumor necrosis factor-α and interleukin-1β), rebalanced levels of short-chain fatty acids and bile acids, improved gut barrier integrity and increased intestinal T regulatory cells. Directed chemical manipulation provides an additional tool for deciphering the chemical biology of the gut microbiome and might advance microbiome-targeted therapeutics.
2019-12-31 | GSE104913 | GEO
Project description:Microbiome DNA from the adhering fraction of a sheep rumen. The RSTs were generated using an improved version of SARST (referred to as iSARST) from the microbiome DNA extracted from the adhering fraction of the rumen content taken from a sheep. The iSARST method is going to be submitted to Nature Biotechnology for publication. Keywords: other
2004-10-15 | GSE1842 | GEO
Project description:The host and microbiome coexist in the natural environment, engaging in constant and dynamic interactions that are fundamental to human health. Understanding these interactions requires unbiased, spatially resolved techniques capable of mapping gene expression in both the host and microbiome within their native microenvironment. In this study, we introduce microDBiT, a spatial sequencing technology that enables simultaneous, high-resolution profiling of both host and bacterial RNAs (mRNA and rRNA). By applying microDBiT, we successfully mapped the spatial distribution of host and bacterial RNAs in the intestines, colon, and tongue of germ-free (GF) mice colonized with bacteria. Furthermore, we utilized this approach in a dextran sulfate sodium (DSS)-induced colitis model, uncovering key mRNA-level changes in both host and microbial cells. This study demonstrates the power of microDBiT in resolving host-microbiome interactions at the transcriptomic level, providing critical insights into host-pathogen dynamics, immune responses, and microbiome-driven disease mechanisms.
2026-04-01 | GSE294814 | GEO
Project description:Investigation of the cellular adjustments required for life on air by M. gorgona MG08, M. rosea SV97, and M. palsarum NE2 by comparing the proteomes of the three strains when exposed to air (~1.9 p.p.m.v. CH4) and when exposed to high CH4 concentrations (~1000 p.p.m.v. CH4) in air
2024-03-20 | PXD046190 | Pride
Project description:Integrating air microbiome for comprehensive air quality analysis
| PRJEB76800 | ENA