Project description:Animal nutrition considerably affects milk composition that influences its nutritional quality. Milk component synthesis and secretion by the mammary gland involve the expression of a large number of genes whose nutritional regulation remains poorly defined. In this study, 16 lactating goats received 4 experimental diets differing in either forage to concentrate ratio (high forage, HF, or low forage, LF) supplemented, or not, with lipids (whole rapeseeds, RS, or sunflower oil, SO) in a 4 x 4 Latin Square design. To investigate the pathways regulated by nutrition, we examined the effect of these diets on the expression of approximately 8400 genes in caprine mammary gland using a bovine oligonucleotide microarray.
Project description:Animal nutrition considerably affects milk composition that influences its nutritional quality. Milk component synthesis and secretion by the mammary gland involve the expression of a large number of genes whose nutritional regulation remains poorly defined. In this study, 16 lactating goats received 4 experimental diets differing in either forage to concentrate ratio (high forage, HF, or low forage, LF) supplemented, or not, with lipids (whole rapeseeds, RS, or sunflower oil, SO) in a 4 x 4 Latin Square design. To investigate the pathways regulated by nutrition, we examined the effect of these diets on the expression of approximately 8400 genes in caprine mammary gland using a bovine oligonucleotide microarray. Due to the limited quantity of mammary RNA available, equal amounts of total RNA sample from mammary gland of each goat belonging to the same Latin Square group were mixed together before labeling. Each mammary pooled sample (4 by dietary treatment) was then co-hybridized with a standard reference corresponding to a mixture of purified total RNA from several caprine tissues. Each hybridization was repeated in a dye-swap manner for a total of 32 slides (8 slides and 4 independent comparisons by dietary treatment).
Project description:Twelve midlactation cows received 4 diets differing in forage-to-concentrate ration (High (HF) versus Low (LF) forage supplemented or not with lipids (HF with whole intact rapeseeds (HF-RS) and LF with sunflower oil (LF-SO))
Project description:Twelve midlactation cows received 4 diets differing in forage-to-concentrate ration (High (HF) versus Low (LF) forage supplemented or not with lipids (HF with whole intact rapeseeds (HF-RS) and LF with sunflower oil (LF-SO)) 12 cows got into 4 groups, each cow was received 4 different diets in a latin square design Green*txt and Red_*txt raw data files contain Cy3 and Cy5 signal intensities, respectively.