Project description:Petroleum hydrocarbons are recalcitrant contaminants, which has caused most serious environmental problems. Acinetobacter calcoaceticus Aca13 was isolated from petroleum polluted soil for petroleum biodegradation. Hexadecane and naphthalene were used to incubate with Acinetobacter calcoaceticus Aca13. After incubation, the whole transcriptome was obtained from treated groups and control groups, and then used for RNA sequence and analysis. Obtained data in this project will help us understand the biodegradation mechanism of hexadecane and naphthalene, and will be helpful for the bioremediation of petroleum hydrocarbons.
Project description:<p>As the petrochemical industry continues to advance, the exacerbation of ecological imbalance and environmental degradation due to petroleum pollution is increasingly pronounced. The synergistic interaction between plants and microorganisms are pivotal in the degradation of petroleum hydrocarbons; however, the underlying degradation mechanisms are not yet fully understood. In this study, we employed a multi-omics approach, integrating transcriptomics, 16S rRNA gene sequencing and metabolomics, to analyze key differential genes, dominant microbial strains and root-secreted metabolites involved in petroleum hydrocarbon degradation in alfalfa. The results show that several stress-related genes are upregulated in alfalfa contaminated with petroleum hydrocarbon. Pseudomonas, Rhodococcus and Brevundimonas remain dominant species in the rhizosphere microbiome. Additionally, when applying pantothenic acid, malic acid and citric acid selected from metabolomics to the oil sediment used for growing alfalfa, the degradation rates significantly improved, with pantothenic acid application resulting in approximately a 10% higher degradation rate compared to other treatments. Our findings offer essential insights for optimizing phytoremediation strategies and improving the efficiency of soil decontamination</p>
Project description:Rhizosphere and bulk soil contaminated with petroleum hydrocarbons. 16s amplicon seq.
| PRJNA642957 | ENA
Project description:The Biodegradation of Petroleum Hydrocarbons in Soils Co-Contaminated by Petroleum Hydrocarbons and Petroleum Hydrocarbon Derived Heavy Metals