Project description:We present a label free proteome dataset of the vascular sap proteome of three commercially important Eucalyptus species (Eucalyptus camaldulensis, Eucalyptus grandis and Eucalyptus urophylla). Protein extraction from the vascular system was carried out using a pressure bomb, in solution digested and peptides were analyzed using a Q-Exactive instrument. Protein identification was carried out using stringent database searches and only in silico predicted extracellular proteins were considered as part of the sap proteome. The results here described can be used as a reference for the proteome sap analysis of Eucalyptus plants grown under different conditions.
Project description:This SuperSeries is composed of the following subset Series: GSE24130: Gene expression in xylem tissue on an Eucalyptus pseudo-testcross population: discovery array probes GSE24195: Gene expression in xylem tissue on an Eucalyptus pseudo-testcross population: genotyping subset of discovery array probes Refer to individual Series
Project description:The daily cycle of night and day affects the behaviour and physiology of almost all living things. At the molecular level, many genes show daily changes in expression levels. To determine whether changes in transcript abundance occur in wood forming tissues of Eucalyptus trees we used a cDNA microarray to examine gene expression levels at roughly four hour intervals throughout the day. Experiments were performed using RNA extracted from two biological replicates - GU (Eucalyptus grandis x E. urophylla) and GC (Eucalyptus grandis x camaldulensis) trees. A loop design was used, linking six time points. A dye swap was incorporated to eliminate dye bias.
Project description:The expression pattern of transcripts involved in secondary development was determined in four genotypes of E. tereticornis using 8x60K Gene chip. The genome-wide expression data was used to develop a gene co-expression network for xylogenesis. Agilent one-color experiment, Organism: Eucalyptus,Custom Agilent Eucalyptus 8x60k Microarray Gene expression (AMADID: 59849 ) designed by Genotypic Technology Pvt.Ltd.
Project description:Illumina HiSeq technology was used to generate mRNA profiles from in vitro Eucalyptus grandis roots interacting with two different Pisolithus microcarpus strains (SI-9 and SI-12) and under two different CO2 concentrations (400 and 650 ppm) . Control roots or ectomycorrhizal root tips were harvested after 1 month and used for RNA extraction. Paired-end (2X150bp) reads were generated and aligned to Eucalyptus grandis transcripts (http://www.phytozome.net/; primarytranscripts only) using CLC Genomics Workbench 6.
Project description:Eucalyptus grandis e Eucalyptus globulus are among the most widely cultivated trees, differing in lignin composition and plantation areas. As temperature is a key modulator in plant metabolism, a large-scale proteome analysis was carried out to investigate changes induced in plantlets grown at different temperatures.
