Project description:The purpose of this study is to explore the influence of the morpho-physiological of Sinapis alba L in response to cadmium challenge
Project description:Cortical neurons exhibit astounding diversity in gene expression as well as in morphological and electrophysiological properties. Most existing neural taxonomies are based on either transcriptomic or morpho-electric criteria, as it has been technically challenging to study both aspects of neuronal diversity in the same set of cells. Here we used Patch-seq to combine patch-clamp recording, biocytin staining, and single-cell RNA sequencing of over 1300 neurons in adult mouse motor cortex, providing a comprehensive morpho-electric annotation of almost all transcriptomically defined neural cell types. We found that, although broad families of transcriptomic types (Vip, Pvalb, Sst, etc.) had distinct and essentially non-overlapping morpho-electric phenotypes, individual transcriptomic types within the same family were not well-separated in the morpho-electric space. Instead, there was a continuum of variability in morphology and electrophysiology, with neighbouring transcriptomic cell types showing similar morpho-electric features, often without clear boundaries between them. Our results suggest that neural types in the neocortex do not always form discrete entities. Instead, neurons follow a hierarchy consisting of distinct non-overlapping branches at the level of families, but can form continuous and correlated transcriptomic and morpho-electrical landscapes within families.
Project description:Climate change is anticipated to exacerbate environmental stressors such as drought, elevated temperatures, and increased CO2 levels, posing a significant threat to crop productivity. We investigated the morpho-physiological acclimation and transcriptional responses of hexaploid wheat (Triticum aestivum L.) under both single and combined stress conditions. Wheat plants were subjected to drought (D), elevated temperature (eT), and elevated CO2 (eC) individually, as well as in double (eT+D, eC+D, eC+eT) and triple (eC+eT+D) stress combinations. Our findings reveal that the eT+D combination resulted in the most severe reductions in growth and yield, while elevated CO2 cccccpartially mitigated these effects by enhancing biomass production and water-use efficiency. Transcriptomic analyses identified key regulatory networks, including specific protein-coding genes, transcription factor (TF) families, and potential marker genes associated with stress adaptation. Overall, this study provides new insights into the complex genetic and physiological mechanisms underlying wheat resilience to multifactorial environmental stress. These findings highlight valuable molecular targets for breeding strategies aimed at improving wheat tolerance to climate change-induced stress conditions
Project description:Primary objectives: The primary objective is to investigate circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
Primary endpoints: circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
| 2533916 | ecrin-mdr-crc
Project description:Genome sequencing of tropical Morpho species
