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METTL14 regulates chondrogenesis through the GDF5–RUNX–extracellular matrix gene axis during limb development [scRNA-seq]

ABSTRACT: METTL14 regulates chondrogenesis through the GDF5–RUNX–extracellular matrix gene axis during limb development [scRNA-seq]

PROVIDER: PRJNA1064080 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27533894.fastq.gz	Fastqsanger.gz
	SRR27533895.fastq.gz	Fastqsanger.gz
	SRR27533896.fastq.gz	Fastqsanger.gz
	SRR27533897.fastq.gz	Fastqsanger.gz

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METTL14 regulates chondrogenesis through the GDF5–RUNX–extracellular matrix gene axis during limb development [m6A-seq]

Project description:METTL14 regulates chondrogenesis through the GDF5–RUNX–extracellular matrix gene axis during limb development [m6A-seq]

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METTL14 regulates chondrogenesis through the GDF5–RUNX–extracellular matrix gene axis during limb development [RNA-seq]

Project description:METTL14 regulates chondrogenesis through the GDF5–RUNX–extracellular matrix gene axis during limb development [RNA-seq]

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METTL14 regulates chondrogenesis through the GDF5–RUNX–extracellular matrix gene axis during limb development [scRNA-seq]

Project description:Mettl14 forms a protein complex that induces m6A methylation on RNA but its roles in mouse limb development remains elusive. Here, the Mettl14 gene was knocked out in a limb bud-specific manner in Mettl14 flox/flox mice with the Prx1 promoter-driven cre. Dissociated cells from day 12.5 (E12.5) embryonic limb buds were applied to scRNA-seq to identify differentially expressed genes in a cell type-specific manner. Samples prepared from Per1cre;Mettl14 wild type/flox and Per1cre;Mettl14 flox/flox mice were labeled as cHet and cKO, respectively.

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METTL14 regulates chondrogenesis through the GDF5–RUNX–extracellular matrix gene axis during limb development [RNA-seq]

Project description:METTL14 forms a protein complex that induces m6A methylation on RNA but its roles in mouse limb development remains elusive. We knocked out Mettl14 in a limb bud-specific manner with the Prx1 promoter-driven Cre. The resulting Prx1-cre;Mettl14 flox/flox (conditional KO or cKO) mice showed shorter limbs with disrupted bone and cartilage development compared with the heterozygous Prx1-cre;Mettl14 wild type/flox (cHet) control mice. To understand the molecular basis for the abnormalities, we analyzed polysome RNA prepared from day 12.5 (E12.5) embryonic limb buds with RNA-seq.

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METTL14 regulates chondrogenesis through the GDF5–RUNX–extracellular matrix gene axis during limb development [m6A-seq]

Project description:METTL14 forms a protein complex that induces m6A methylation on RNA but its roles in mouse limb development remains elusive. We knocked out Mettl14 in a limb bud-specific manner with the Prx1 promoter-driven Cre. The resulting Prx1-cre;Mettl14 flox/flox (conditional KO or cKO) mice showed shorter limbs with disrupted bone and cartilage development compared with the heterozygous Prx1-cre;Mettl14 wild type/flox (cHet) control mice. To understand the molecular basis for the abnormalities, we applied total RNA from day 12.5 (E12.5) embryonic limb buds to m6A-seq.

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Roles of Mettl14 in mouse limb bud development

Project description:m6A regulates virtually every step in RNA metabolism. However, its toles in limb development remains largely unknown. To understand the roles, we created a limb bud-specific conditional knockout (cKO) mice and control heterozygous (cHet) mice of the Mettl14 gene, which encodes an essential subunit in the m6A methyltransferase complex METTL3/METTL14. We harvested limb buds from the mice on E12.5 and applied the proteins to quantitative mass spectrometry to understand how the depletion of Mettl14 affected the proteomes.

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Influence of DNA-methylation at multiple stages of limb chondrogenesis

Project description:Influence of DNA-methylation at multiple stages of limb chondrogenesis

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