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Ribo– RNA-seq of psoriasis mouse model delivered (i.v.) EPIC by LNP at D1.5

ABSTRACT: Ribo– RNA-seq of psoriasis mouse model delivered (i.v.) EPIC by LNP at D1.5

PROVIDER: PRJNA1065373 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27562101_1.fastq.gz	Fastqsanger.gz
	SRR27562101_2.fastq.gz	Fastqsanger.gz
	SRR27562102_1.fastq.gz	Fastqsanger.gz
	SRR27562102_2.fastq.gz	Fastqsanger.gz

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Ribo– RNA-seq of psoriasis mouse model delivered (i.v.) EPIC by LNP at D1.5

Project description:Efforts to advance RNA aptamers as a novel therapeutic modality have been limited by their susceptibilty to degradation and immunogenicity. In a previous study, we demonstrated synthesized double-stranded circular RNAs (ds-cRNAs) with minimal immunogenicity targeted to dsRNA-activated Protein Kinase R (PKR). Here, we test the therapeutic potential of ds-cRNAs in a mouse model of imiquimod-induced psoriasis. We find that genetic supplementation of ds-cRNAs leads to inhibition of PKR, resulting in alleviation of downstream interferon alpha (IFNα)/dsRNA signals and attenuation of psoriasis phenotypes. Delivery of ds-cRNAs by lipid nanoparticles to the spleen attenuates PKR activity in examined splenocytes, resulting in reduced epidermal thickness. These findings suggest that ds-cRNAs represent a promising approach to mitigate excessive PKR activation for therapeutic purposes.

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