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FoxO transcription factors actuate the formative pluripotency specific gene expression programme

ABSTRACT: FoxO transcription factors actuate the formative pluripotency specific gene expression programme

PROVIDER: PRJNA1065862 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27595409.fastq.gz	Fastqsanger.gz
	SRR27595410.fastq.gz	Fastqsanger.gz
	SRR27595411.fastq.gz	Fastqsanger.gz
	SRR27595412.fastq.gz	Fastqsanger.gz
	SRR27595413.fastq.gz	Fastqsanger.gz

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FoxO transcription factors actuate the formative pluripotency specific gene expression programme [RNA-Seq]

Project description:Naïve pluripotency is sustained by a self-reinforcing gene regulatory network (GRN) comprising core and naïve pluripotency-specific transcription factors (TFs). Upon exiting naïve pluripotency, ES cells transition through a formative post-implantation-like pluripotent state. However, the mechanisms underlying disengagement from the naïve GRN and initiation of the formative GRN remain unclear. Here, we demonstrate that phosphorylated AKT acts as a gatekeeper that prevents nuclear localization of FoxO TFs in naïve ESCs. PTEN-mediated reduction of AKT activity allows nuclear entry by FoxO TFs, enforcing a cell fate transition by binding and activating formative pluripotency-specific enhancers. Indeed, FoxO TFs are necessary and sufficient for transition from the naïve to the formative pluripotent state. Our work uncovers a pivotal role for FoxO TFs and AKT signalling in mechanisms underlying the exit from naïve pluripotency, a critical early embryonic cell fate transition.

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FoxO transcription factors actuate the formative pluripotency specific gene expression programme [ATAC-Seq]

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FoxO transcription factors actuate the formative pluripotency specific gene expression programme [ChIP-Seq]

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FoxO transcription factors actuate the formative pluripotency specific gene expression programme [ChIP-Seq]

Project description:FoxO transcription factors actuate the formative pluripotency specific gene expression programme [ChIP-Seq]

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FoxO transcription factors actuate the formative pluripotency specific gene expression programme [RNA-Seq]

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FoxO transcription factors actuate the formative pluripotency specific gene expression programme [ATAC-Seq]

Project description:FoxO transcription factors actuate the formative pluripotency specific gene expression programme [ATAC-Seq]

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FoxO transcription factors actuate the formative pluripotency specific gene expression programme.

Project description:Naïve pluripotency is sustained by a self-reinforcing gene regulatory network (GRN) comprising core and naïve pluripotency-specific transcription factors (TFs). Upon exiting naïve pluripotency, embryonic stem cells (ESCs) transition through a formative post-implantation-like pluripotent state, where they acquire competence for lineage choice. However, the mechanisms underlying disengagement from the naïve GRN and initiation of the formative GRN are unclear. Here, we demonstrate that phosphorylated AKT acts as a gatekeeper that prevents nuclear localisation of FoxO TFs in naïve ESCs. PTEN-mediated reduction of AKT activity upon exit from naïve pluripotency allows nuclear entry of FoxO TFs, enforcing a cell fate transition by binding and activating formative pluripotency-specific enhancers. Indeed, FoxO TFs are necessary and sufficient for the activation of the formative pluripotency-specific GRN. Our work uncovers a pivotal role for FoxO TFs in establishing formative post-implantation pluripotency, a critical early embryonic cell fate transition.

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FoxO transcription factors actuate the formative pluripotency specific gene expression programme

Project description:This SuperSeries is composed of the SubSeries listed below.

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