Project description:In response to the issues of low denitrification efficiency and high N₂O emissions in the biological nitrogen removal process of low C/N municipal wastewater, studies typically address these challenges by adding carbon sources. In this study, the addition of microorganisms enhanced the carbon flow and electron transport for nitrate reduction, significantly improving the denitrification performance of low C/N wastewater and reducing N₂O production. Proteomic analysis was employed to explore the mechanisms underlying this effect. The results revealed that the metabolites produced by the added microorganisms, S. oneidensis MR-1 and B. subtilis, including biosurfactants, heme, and cytochromes, altered the intracellular carbon redistribution in P. denitrificans, leading to an increased carbon flow directed towards nitrate reduction, thus enhancing total nitrogen removal efficiency.
2024-10-15 | PXD056799 |
Project description:Integrated evaluation for advanced removal of nitrate using novel solid carbon Biochar/corncob/PHBV composite
| PRJNA1181957 | ENA
Project description:Integrated evaluation for advanced removal of nitrate using novel solid carbon Biochar/corncob/PHBV composite
Project description:Nitrogen (N) is a key macronutrient required for plant growth, development, and yield. Improving nitrogen use efficiency (NUE) in maize is important for sustainable crop production and reducing fertilizer-associated environmental impacts. To investigate transcriptional responses to nitrate availability, we examined genome-wide gene expression dynamics in maize under nitrate-limiting, and nitrate-recovery conditions. This dataset captures rapid transcriptional shifts in both roots and leaves during nitrate deprivation and subsequent nitrate resupply.
2025-12-30 | GSE312408 | GEO
Project description:Nitrate removal and phenol degradation
| PRJNA1195333 | ENA
Project description:Enhanced simultaneous organics and nutrients removal in tidal flow constructed wetland using activated alumina as substrate treating domestic wastewater
Project description:Vitamin-D3 (VitD3) exhibits pleiotropic effects in host’s physiology by acting on several organs. To mechanistically understand how VitD3 influences the colon physiology we interrogated the gene expression profile of mouse colon in response to high or low VitD3 bioavailability. VitD3 tissue bioavailability was modulated in mice with the use of diets supplemented with different concentrations of VitD3 (0, 2 or 10 IU/g) and the use of Gc knockout mouse strain in where VitD3 bioavailability is enhanced. Vitamin-D3 (VitD3) exhibits pleiotropic effects in host’s physiology by acting on several organs. To mechanistically understand how VitD3 influences the colon physiology we interrogated the gene expression profile of mouse colon in response to high or low VitD3 bioavailability. VitD3 tissue bioavailability was modulated in mice with the use of diets supplemented with different concentrations of VitD3 (0, 2 or 10 IU/g) and the use of Gc knockout mouse strain in where VitD3 bioavailability is enhanced.
Project description:Acidification of groundwater co-occurring with nitrate pollution is a common, global environmental health hazard. Denitrifying bacteria have been leveraged for the in-situ removal of nitrate in groundwater. However, co-existing stressors—like low pH—reduce the efficacy of these biological removal processes. Castellaniella sp. str. MT123 is a complete denitrifier that was isolated from acidic, nitrate-contaminated groundwater. The strain grows robustly by nitrate respiration at pH < 6.0 while completely reducing nitrate to dinitrogen gas. Genomic analyses of MT123 revealed few previously characterized acid tolerance genes. Thus, we utilized a combination of proteomics, metabolomics, and competitive mutant fitness to characterize the genetic mechanisms of MT123 acclimation to growth under mildly acidic conditions. We found that glutamate accumulation is critical in the acid acclimation of MT123, likely through its decarboxylation to GABA. This is despite the fact that MT123 lacks the canonical glutamate decarboxylase-glutamate/GABA antiporter system implicated in acid tolerance in other bacteria. Additionally, branched chain amino acid (BCAA) appears to be detrimental to cell growth at lower pHs. Genetic analysis previously linked MT123 to a population of Castellaniella that bloomed—concurrent to nitrate removal—during a biostimulation effort to reduce groundwater nitrate concentrations at MT123’s location of origin. Thus, our analyses provide novel insight into mechanisms of acclimation to acidic conditions in a strain with significant potential for nitrate bioremediation.