Dataset Information

Co-culture of polyps and Bacillus paramycoides SG15

ABSTRACT: Co-culture of polyps and Bacillus paramycoides SG15 Raw sequence reads

PROVIDER: PRJNA1067197 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	SRR27674203_1.fastq.gz	Fastqsanger.gz
	SRR27674203_2.fastq.gz	Fastqsanger.gz
	SRR27674204_1.fastq.gz	Fastqsanger.gz
	SRR27674204_2.fastq.gz	Fastqsanger.gz
	SRR27673705_subreads.fastq.gz	Fastqsanger.gz

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Project description:Symbiotic microorganisms play an important role in the growth and development of marine invertebrates, such as the sea moon, affecting their metamorphosis process. The outbreak of jellyfish puts pressure on marine ecosystems and affects their stability. However, researches on the development of jellyfish are still fewer, the use of biological control of jellyfish disaster is still in the theoretical stage, and the inhibition mechanism of microorganisms on jellyfish remains to be revealed. We isolated a strain of Bacillus paramycoides SG15 from rhizosphere sediments of seagrass Zostera japonica, and found that this strain could inhibit the asexual reproduction of jellyfish Aurelia coerulea polyp larva. Through co-culture experiments, we used transcriptomics, metabolomics and fluorescence in situ hybridization techniques, combined with the genome and metabolome of SG15, and found that this isolate could inhibit the asexual reproduction rate of the larva polyp by inhibiting the absorption of vitamins, and the endoderm cells were the most important action site of SG15. Although there are relatively high concentrations of vitamins and their derivatives in the surrounding environment, the transport of hydra is reduced, and most vitamins cannot be synthesized in the polys as well, thus affecting the growth and reproduction of polyps. Our study, analyzed the interaction processes between Bacillus and polyps and revealed the inhibiting mechanism of the polyp asexual reproduction by B. paramycoides SG15, which laid a theoretical foundation for the subsequent analysis of interactions between jellyfish and microorganisms, bring materials for the biological control of jellyfish disasters. Polyps culture media is reported in the current study <a href='https://www.ebi.ac.uk/metabolights/MTBLS9419' rel='noopener noreferrer' target='_blank'>MTBLS9419</a>.Polyps whole organism is reported in <a href='https://www.ebi.ac.uk/metabolights/MTBLS9409' rel='noopener noreferrer' target='_blank'>MTBLS9409</a>.

Project description:Symbiotic microorganisms play an important role in the growth and development of marine invertebrates, such as the sea moon, affecting their metamorphosis process. The outbreak of jellyfish puts pressure on marine ecosystems and affects their stability. However, researches on the development of jellyfish are still fewer, the use of biological control of jellyfish disaster is still in the theoretical stage, and the inhibition mechanism of microorganisms on jellyfish remains to be revealed. We isolated a strain of Bacillus paramycoides SG15 from rhizosphere sediments of seagrass Zostera japonica, and found that this strain could inhibit the asexual reproduction of jellyfish Aurelia coerulea polyp larva. Through co-culture experiments, we used transcriptomics, metabolomics and fluorescence in situ hybridization techniques, combined with the genome and metabolome of SG15, and found that this isolate could inhibit the asexual reproduction rate of the larva polyp by inhibiting the absorption of vitamins, and the endoderm cells were the most important action site of SG15. Although there are relatively high concentrations of vitamins and their derivatives in the surrounding environment, the transport of hydra is reduced, and most vitamins cannot be synthesized in the polys as well, thus affecting the growth and reproduction of polyps. Our study, analyzed the interaction processes between Bacillus and polyps and revealed the inhibiting mechanism of the polyp asexual reproduction by B. paramycoides SG15, which laid a theoretical foundation for the subsequent analysis of interactions between jellyfish and microorganisms, bring materials for the biological control of jellyfish disasters. Polyps whole organism is reported in the current study <a href='https://www.ebi.ac.uk/metabolights/MTBLS9409' rel='noopener noreferrer' target='_blank'>MTBLS9409</a>.Polyps culture media is reported in <a href='https://www.ebi.ac.uk/metabolights/MTBLS9419' rel='noopener noreferrer' target='_blank'>MTBLS9419</a>.

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Co-culture of polyps and Bacillus paramycoides SG15

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