Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of PXR on gene expression in LS174T cells

ABSTRACT: Effect of PXR on gene expression in LS174T cells

PROVIDER: PRJNA1067556 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR27678979_1.fastq.gz	Fastqsanger.gz
	SRR27678979_2.fastq.gz	Fastqsanger.gz
	SRR27678980_1.fastq.gz	Fastqsanger.gz
	SRR27678980_2.fastq.gz	Fastqsanger.gz
	SRR27678981_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

Effect of PXR on gene expression in LS174T cells

Project description:To investigate the role of PXR in regulating RNA expression level and stability in LS174T cells

2025-04-28 | GSE253843 | GEO

Enhanced CLIP-Seq (eCLIP-Seq) of mouse Pxr in LS174T cells

Project description:Enhanced CLIP-Seq (eCLIP-Seq) of mouse Pxr in LS174T cells

| PRJNA1194536 | ENA

Enhanced CLIP-Seq (eCLIP-Seq) of mouse Pxr in LS174T cells

Project description:eCLIP-seq identified mouse Pxr as an RNA-binding protein to interact with mature mRNAs

2025-04-28 | GSE283561 | GEO

Pregnane X-receptor (PXR/NR1I2) activity is a hallmark of cancer stem cells and drives post-treatment tumor recurrence in colon cancer

Project description:Post-treatment recurrence remains a major clinical challenge in colorectal cancer (CRC), and increasing evidence suggests that Cancer Stem Cells (CSCs) may mediate this process due to their enhanced resistance to conventional therapeutics. We hypothesized that the Pregnane-X receptor (PXR) may drive the chemoresistance of colorectal CSCs.. shRNA-mediated PXR down-regulation was used to analyze the role of PXR on self-renewal in the colorectal cancer cell Line LS174T. Expression and activity of PXR were quantified under conditions that promote Cancer Stem Cells (CSCs) enrichment. Conversely, CSC characterization was performed in cells enriched along their PXR activity level.

2016-08-01 | E-MTAB-3471 | biostudies-arrayexpress

Change of gene expression of mutation of TP53 in LS174T cells

Project description:Change of gene expression of mutation on TP53 exon3 or exon10 by CRISPR-Cas9 system in LS174T cells

2018-12-26 | GSE124349 | GEO

Gene expression profile of ATF6a and ATF6b overexpression in LS174T cells

Project description:To further development of our mRNA expression approach to ER stress, we have employed whole genome microarray expression profiling as a discovery platform to identify ER stress-responsible genes. LS174T cells were overexpressed with ER stress mediators, ATF6a or ATF6b. Genes responsible for each mediator were extracted and categorized by Gene Ontology. ATF6a and ATF6b were overexpressed in LS174T. Subsequently, cells were treated with 2 mM sodium butyrate, which induces LS174T cells to differentiate to mature goblet cells. Genes responsible for each mediator were extracted and categorized by Gene Ontology in GeneRanker program of Genomatix platform.

2015-08-05 | E-GEOD-49567 | biostudies-arrayexpress

Sequencing of HepaRG cells with functional knockout of PXR

Project description:Purpose: To assess effect of PXR functional knockout

2022-02-10 | GSE149001 | GEO

Gene expression profile of ATF6a and ATF6b overexpression in LS174T cells

2015-08-05 | GSE49567 | GEO

Change of gene expression of mutation of TP53 in LS174T cells

Project description:Change of gene expression of mutation of TP53 in LS174T cells

| PRJNA511762 | ENA

Gene expression profile upon IL-22 stimulation, forced expression of Hes1, or IL-22 stimulation under forced expression of Hes1, in human colon carcinoma-derived LS174T cells

Project description:A human colon carcinoma-derived cell line LS174T was modified to overexpress Hes1, a bHLH-type transcription factor, upon doxycycline addition (designated as LS174T-tetON-Hes1 cells), using the T-rex system (Invitrogen). We have previously shown that these cells can overexpress Hes1 under the control of CMV promoter (Zheng et al, Inflamm Bowel Dis, 17;2251-2260, 2011), and the amont of the overexpressed Hes1 protein reaches to the maximal level in early as 3 hours from doxycycline addition (100ng/ml), which persists for up to 24 hours. In the present experiment, LS174T-tetON-Hes1 cells were treated with recombinant human IL-22 (20ng/ml) alone, doxycycline alone (100ng/ml), co-treated by both IL-22 and doxycycline, or left untreated (Control) for 24 hours, and subjected for analysis. Experiment was done using a modified sub-line of LS174T cell (LS174T-tetON-Hes1 cells), in which overexpression of Hes1 can be induced by a Doxycycline dependent manner.

2013-10-01 | GSE43012 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data