Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The effects of HSP60 chaperone deficiency on zebrafish larvae

ABSTRACT: The effects of HSP60 chaperone deficiency on zebrafish larvae

PROVIDER: PRJNA1068542 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR27715729_1.fastq.gz	Fastqsanger.gz
	SRR27715729_2.fastq.gz	Fastqsanger.gz
	SRR27715730_1.fastq.gz	Fastqsanger.gz
	SRR27715730_2.fastq.gz	Fastqsanger.gz
	SRR27715731_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

The effects of HSP60 chaperone deficiency on zebrafish larvae

2024-09-27 | GSE254091 | GEO

The proteomics effects of HSP60 chaperone deficiency in zebrafish larvae

Project description:To understand the effects of Hsp60 deficiency in developing vertebrates, we generated CRISPR/Cas9-mediated hspd1 knockout zebrafish lines by targeting exon 2 to induce a frameshift mutation. We selected an allele with a 56 base pair deletion inducing a frameshift mutation leading to loss of protein functions. We examined the proteome changes in zebrafish larvae at 5 days post fertilization (DPF). Wildtype control and hspd1-/- larvae at 5dpf, were analyzed by TMT and nanoLC-MS/MS based proteomcis. For this purpose, we studied five pools from each genotype, and each pool consisted of five larvae.

2024-09-27 | PXD048920 | Pride

Modelling HSP60 deficiency using an inducible transgenic HSP60 ATPase deficient mutant

Project description:To investigate the immediate effect of HSP60 dysfunction on mitochondrial functions, we induced the expression of a dominant-negative ATPase-deficient HSP60 mutant (HSP60-D423A) in HEK293 cells using tetracycline . Incorporation of HSP60-D423A subunits into HSP60 heptamer rings leads to dysfunction of the chaperonin complex. We titrated tetracycline levels to have a biologically relevant cellular model that can be monitored over time. Expression of the HSP60-D432A protein resulted in significantly reduced cell counts at 72 hours induction compared to uninduced and HSP60-WT co-expressing cells. To study the effects of onset of HSP60 deficiency, we monitored transcriptional changes using RNASeq.

2024-09-27 | GSE254495 | GEO

Proteomic mapping of fibroblasts from patients with HSP60 deficiency

Project description:Deficiency of the mitochondrial chaperone HSP60 has detrimental effects on the mitochondrial functions, and can in patient’s lead to hypomyelination disorder. The present study investigates the proteome in cultivated skin fibroblasts originating from two patients with HSP60 genetic variants and hypomyelination.

2025-09-11 | PXD048923 | Pride

Modelling HSP60 deficiency using an inducible transgenic HSP60 ATPase deficient mutant

Project description:Modelling HSP60 deficiency using an inducible transgenic HSP60 ATPase deficient mutant

| PRJNA1070667 | ENA

Proteomics effects of HSP60 deficiency in HEK cells, using an inducible transgenic HSP60 ATPase deficient mutant.

2025-05-06 | PXD048918 | Pride

Zebrafish larvae response to AgNPs and AgNO3

Project description:Transcriptional profiling of zebrafish larvae comparing control with AgNO3 or AgNPs exposed zebrafish larvae.

2015-09-01 | GSE61186 | GEO

3dpf untreated zebrafish larvae vs. 3dpf zebrafish larvae treated with 20mM PTZ for 30 minutes and 90 minutes

Project description:Transcriptional profiling of 3dpf wild type zebrafish larvae treated with 20mM PTZ for 30 and 90 minutes compared with 3dpf wild type control untreated zebrafish larvae.

2017-02-24 | GSE95312 | GEO

Proteomics analysis of behavioral adaptation in zebrafish larvae

Project description:In this work we investigated how the brain proteome of the larval zebrafish is modified by behavioral adaptation to the environmental challenge of a water vortex. We monitored the behavior of larvae and observed that they behaviorally adapted to the presence of a water vortex. We obtained the larval zebrafish brain proteome by extracting brains from zebrafish larvae and analyzing them using and LFQ-based LC-MS/MS-approach. In total we identified 5929 proteins in the larval brain. Within this proteome, we identified 57 proteins that were significantly regulated following experience of the water vortex: 41 proteins were up regulated and 16 were down regulated. Of these, 29 proteins are known to have neuronal functions, 17 proteins are known to have other cellular functions, and 11 proteins are still uncharacterized.

2019-05-24 | PXD011981 | Pride

Expression profiles of E. coli groESL(-) cells expressing human Hsp60(wt)/Hsp10 or Hsp60-(p.Val98Ile)/Hsp10 operons

Project description:To identify regulatory responses that could give clues as to the cause(s) of the growth arrest observed in E. coli cells expressing mutant Hsp60-(p.Val98Ile) and Hsp10 versus cells expressing wild type Hsp60 and Hsp10, we performed microarray analyses. We reasoned that the decreased chaperonin activity of the Hsp60-(p.Val98Ile) protein would greatly impair or abolish folding of E. coli proteins that depend on chaperonin assistance. The resulting decreased levels of various activities may cause compensatory up-regulation or deregulation of genes. The transcriptomes of cells shifted to expression of the arabinose-inducible chaperonin operon with either the Hsp60-(p.Val98Ile) mutant or the wild type Hsp60 from the second plasmid were compared. RNA samples from three independent cultures expressing the mutant or two independent cultures expressing wild type Hsp60 were pooled, respectively. Keywords: Comparison of cells expressing two different variants of Hsp60

2008-04-14 | GSE10974 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data