Project description:Regenerative life support systems for space crews recycle organic and inorganic waste into water, food and oxygen using different organisms. For instance, the European Space Agency's MELiSSA uses Limnospira indica PCC8005 for air revitalisation and food production. Before use in space, the components' compatibility with reduced gravity must be tested. This innovative study introduces a novel ground analog designed specifically for microgravity experiments involving cyanobacteria, employing a cutting-edge random positioning machine (RPM). Limnospira indica PCC8005 was shown to grow slower under simulated microgravity and whole proteome analysis revealed a downregulation of e.g. ribosomal proteins, glutamine synthase and nitrate uptake transporters while an upregulation was found for gas vesicle proteins, carboxysome proteins and phycobiliproteins. All together our results suggested that L. indica experienced carbon limitation when cultivated in simulated microgravity conditions.
Project description:Proteomic and lipidomic analysis of Ground Demonstrator Model of ArtEMISS-C (Science Verification Test), MELiSSA Project on Limnospira indica PCC 8005 P3.
Proteomic analysis has been performed with AB Sciex TripleTOF 6600.
Lipidomic analysis has been performed with AB Sciex ZenoTOF 7600.
