Project description:Analysis of gene expression in NIH 3T3 cells stably knockdown for Selt (GenBank accession number NM_001040396) using vector based siRNA technique in comparison to gene expression of vector transfected NIH 3T3 cells.
2009-11-01 | GSE17087 | GEO
Project description:Effect of FGF2 on gene expression of NIH 3T3 fibroblast
Project description:Analysis of Immediate Early Response 2 (Ier2)-inducible NIH 3T3 cells after Ier2 induction with RheoSwitch ligand RSL-1. Results provide insight into the function of Ier2 in NIH 3T3 mouse embryonal fibroblasts. Immediate early genes, including Ier2, are rapidly induced in quiescent cells by proliferation and migration-inducing stimuli. Microarray gene expression profiling was performed to identify differentially expressed genes following overexpression of Ier2 in NIH 3T3-Ier2 inducible cells after 24 hour induction of Ier2.
Project description:Expression profiling of HepG2 human liver carcinoma cells and NIH 3T3 mouse fibroblasts after arsite treatment for 24h. RNA-seq data comprise 4 groups: NIH 3T3 mouse fibroblasts control and arsite treatment, and HepG2 human liver carcinoma cells control and arsenite treatment. Jena Centre for Systems Biology of Ageing - JenAge (www.jenage.de)
Project description:We analyzed the effect on HNF4A loss on Histone modifications (H3K4me1, H3K27ac) by ChIP-seq and CUT&Tag using control and HNF4A KO livers samples. We also examined chromatin accessibility by ATAC-seq in control and HNF4A KO livers. We also performed CUT&Tag and ATAC-seq of mouse fibroblast cells (NIH 3T3) treated with control or HNF4A expression vector.
Project description:Ultraviolet radiation (UV) causes certain side effects to the skin, and their accumulation to a certain extent can lead to accelerated aging of the skin. Recent studies suggests that α-arbutin may be useful in various disorders such as hyperpigmentation disorders, wound healing, and antioxidant activity. However, the role of α-arbutin in skin photodamage is unclear. In this study, under UVA-induced photodamage conditions, α-arbutin treated mouse skin fibroblasts (NIH-3T3) can repair DNA damage and resist apoptosis by reducing the production of reactive oxygen species (ROS) and increasing the phosphorylation of glycogen synthase kinase 3 beta (GSK3β) to orchestra AKT/GSK3β pathway. Meanwhile, α-arbutin can also regulate collagen metabolism and facilitate the replenishment of collagen by targeting the phosphorylation of SMAD3 to mediate the TGFβ/SMAD pathway in NIH-3T3. In conclusion, we found that α-arbutin can mitigate the detrimental effects of skin photodamage induced by UVA irradiation, and provides a theoretical basis for the use of α-arbutin in the treatment of skin photodamage.
Project description:Type of experiment: <br> Our experiments examine gene expression profiles from untreated and mechanical stretching NIH 3T3 cells to gain new insights about the molecular and genetic basis of effect of mechanical stress. <br><br> Experimental factors: <br> We compared differences in gene expression profiles between 0.25% mechanical strain and control factor for NIH 3T3 cells. <br><br> The number of hybridizations performed in the experiment: <br> A total of 4 hybridizations were performed on Agilent One-color microarray platform. <br><br> Number of replicates (Biological or Technical)<br> 3 biological replicates for treated condition <br><br> Hybridization design: <br> All treated NIH 3T3 cells sample RNAs were hybridized against a pooling control RNA sample (see above). Cells were cultured in a humidifier incubator at 37 0C and 5% CO2. After cells were confluent, these cells were subjected to cyclic sinusoidal strain at 0.25 Hz for 60 minutes at a maximum membrane indentation of 1 mm., equal to 0.25% strain. Strain experiments were carried out in a humidified incubator with 5% CO2 at 37°C. Control cells were treated identical, however, not exposed to mechanical stress.
