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RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions [ATAC-seq]

ABSTRACT: RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions [ATAC-seq]

PROVIDER: PRJNA1071505 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27798290_1.fastq.gz	Fastqsanger.gz
	SRR27798290_2.fastq.gz	Fastqsanger.gz
	SRR27798291_1.fastq.gz	Fastqsanger.gz
	SRR27798291_2.fastq.gz	Fastqsanger.gz
	SRR27798292_1.fastq.gz	Fastqsanger.gz

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RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions [RNA_iPSC_diff_Mono]

Project description:Monocytes are important for acute myeloid leukemia (AML), but the influence of long non-coding RNAs (lncRNAs) in this context is unknown. Previously we demonstrated that lncRNA SMANTIS maintains endothelial angiogenic functions and endothelial-monocyte adhesion. Here, the role of SMANTIS in monocytes was examined. SMANTIS was higher expressed in monocytes than in endothelial cells, lost in macrophages, and varied in patients with different AML subtypes. Interaction studies revealed that SMANTIS binds RUNX1, a transcription factor frequently mutated in AML, primarily through its Alu-element. RNA-seq after CRISPR/Cas9-mediated deletion of SMANTIS or RUNX1 revealed an association with cell adhesion and both limited the monocyte adhesion to endothelial cells. Mechanistically, SMANTIS KO reduced RUNX1 genomic binding, and interfered the interaction of RUNX1 with EP300. Collectively, SMANTIS interacts with RUNX1 and limits cell adhesion, which may thus offer a novel therapeutic strategy for AML.

2024-08-05 | GSE254680 | GEO

RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions [RNA_THP1_diff_OCL]

2024-08-05 | GSE254681 | GEO

RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions [RNA_THP1_SMANTIS_RUNX1_KO]

2024-08-05 | GSE254679 | GEO

RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions [CUT&RUN]

2024-08-05 | GSE254674 | GEO

RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions [ATAC-seq]

2024-08-05 | GSE254673 | GEO

RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions [RNA_iPSC_diff_Mono]

Project description:RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions [RNA_iPSC_diff_Mono]

| PRJNA1071500 | ENA

RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions [RNA_THP1_diff_OCL]

Project description:RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions [RNA_THP1_diff_OCL]

| PRJNA1071522 | ENA

RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions [RNA_THP1_SMANTIS_RUNX1_KO]

Project description:RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions [RNA_THP1_SMANTIS_RUNX1_KO]

| PRJNA1071501 | ENA

RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions [CUT&RUN]

Project description:RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions [CUT&RUN]

| PRJNA1071504 | ENA

RUNX1 interacts with lncRNA SMANTIS to regulate monocytic cell functions.

Project description:Monocytes, the circulating macrophage precursors, contribute to diseases like atherosclerosis and asthma. Long non-coding RNAs (lncRNAs) have been shown to modulate the phenotype and inflammatory capacity of monocytes. We previously discovered the lncRNA SMANTIS, which contributes to cellular phenotype expression by controlling BRG1 in mesenchymal cells. Here, we report that SMANTIS is particularly highly expressed in monocytes and lost during differentiation into macrophages. Moreover, different types of myeloid leukemia presented specific SMANTIS expression patterns. Interaction studies revealed that SMANTIS binds RUNX1, a transcription factor frequently mutated in AML, primarily through its Alu-element on the RUNT domain. RNA-seq after CRISPR/Cas9-mediated deletion of SMANTIS or RUNX1 revealed an association with cell adhesion and both limited the monocyte adhesion to endothelial cells. Mechanistically, SMANTIS KO reduced RUNX1 genomic binding and altered the interaction of RUNX1 with EP300 and CBFB. Collectively, SMANTIS interacts with RUNX1 and attenuates monocyte adhesion, which might limit monocyte vascular egress.

| S-EPMC11399395 | biostudies-literature

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