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Human cytomegalovirus infection coopts chromatin organization to modulate TEAD1 transcription factor activity [ChIP-seq]

ABSTRACT: Human cytomegalovirus infection coopts chromatin organization to modulate TEAD1 transcription factor activity [ChIP-seq]

PROVIDER: PRJNA1071593 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27819681.fastq.gz	Fastqsanger.gz
	SRR27819682.fastq.gz	Fastqsanger.gz
	SRR27819683.fastq.gz	Fastqsanger.gz
	SRR27819684.fastq.gz	Fastqsanger.gz
	SRR27819685.fastq.gz	Fastqsanger.gz

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Human cytomegalovirus infection coopts chromatin organization to modulate TEAD1 transcription factor activity

Project description:Human cytomegalovirus infection coopts chromatin organization to modulate TEAD1 transcription factor activity

| PRJNA1071598 | ENA

Human cytomegalovirus infection coopts chromatin organization to modulate TEAD1 transcription factor activity [HiChIP]

Project description:Human cytomegalovirus infection coopts chromatin organization to modulate TEAD1 transcription factor activity [HiChIP]

| PRJNA1071597 | ENA

Human cytomegalovirus infection coopts chromatin organization to modulate TEAD1 transcription factor activity [RNA-Seq]

Project description:Human cytomegalovirus infection coopts chromatin organization to modulate TEAD1 transcription factor activity [RNA-Seq]

| PRJNA1071594 | ENA

Human cytomegalovirus infection coopts chromatin organization to modulate TEAD1 transcription factor activity [ATAC-seq]

Project description:Human cytomegalovirus infection coopts chromatin organization to modulate TEAD1 transcription factor activity [ATAC-seq]

| PRJNA1071590 | ENA

Human cytomegalovirus infection coopts chromatin organization to modulate TEAD1 transcription factor activity [HiChIP]

Project description:Human cytomegalovirus (HCMV) infects up to 80% of the world’s population and is linked to serious morbidity in immunocompromised individuals and newborns. Here, using multiple genome-scale assays and computational approaches, we show that HCMV infection leads to widespread changes in chromatin accessibility and chromatin looping, with hundreds of thousands of human genomic regions affected 48 hours after infection. Integrative analyses reveal that HCMV infection perturbs the Hippo signaling pathway by drastically diminishing TEA domain transcription factor 1 (TEAD1) activity. Chromatin immunoprecipitation experiments confirm extensive concordant loss of TEAD1 binding and H3K27ac active histone marks upon infection. TEAD transcription factors are direct effectors of the Hippo signaling pathway, and our data support a position for TEAD1 at the top of a hierarchy involving key developmental pathways with differential expression subsequent to HMCV infection. Known gene targets of TEAD1, including Cellular Communication Network Factor 1 (CCN1) and Thrombospondin 1 (THBS1), are significantly downregulated with HCMV infection, reflecting diminished TEAD1-mediated activity. HCMV infection reduces TEAD1 binding through four distinct mechanisms: closing of TEAD1-bound chromatin, reduction of Yes1 associated transcriptional regulator (YAP1) and phosphorylated YAP (pYAP1) levels, reduction of TEAD1 transcript and protein levels, and alteration of TEAD1 exon-6 usage. Collectively, these comprehensive genome-scale analyses reveal novel mechanisms induced by HCMV infection.

2024-12-06 | GSE254737 | GEO

Human cytomegalovirus infection coopts chromatin organization to modulate TEAD1 transcription factor activity [ChIP-seq]

2024-12-06 | GSE254736 | GEO

Human cytomegalovirus infection coopts chromatin organization to modulate TEAD1 transcription factor activity [ATAC-seq]

2024-12-06 | GSE254734 | GEO

Human cytomegalovirus infection coopts chromatin organization to modulate TEAD1 transcription factor activity [RNA-Seq]

2024-12-06 | GSE254735 | GEO

Human cytomegalovirus infection coopts chromatin organization to modulate TEAD1 transcription factor activity

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-12-06 | GSE254741 | GEO

TEAD1 condensates are transcriptionally inactive storage sites on the pericentromeric heterochromatin

Project description:TEA domain transcription factor 1 (TEAD1), a Hippo pathway transcription factor important in cellular homeostasis and development, is increasingly implicated in cancer biology. Here, we reveal a novel role for TEAD1 in organizing nuclear condensates, independent of active transcription. Using high-resolution imaging, ChIP-seq, RNA-seq and proximity-based proteomics, we demonstrate that in patient-derived renal cell carcinoma cells, TEAD1 forms micron-sized foci by binding to the heterochromatic pericentromeric regions using its DNA-binding domain. These TEAD1 foci do not mediate transcription but instead serve as depots for excess TEAD1. This contrasts with TEAD1 organization in other genomic regions of both RCC and normal kidney cells, where TEAD1 associates with markers of active transcription. Our findings provide a mechanistic framework for TEAD1’s dual regulatory roles, offering new insights into its contribution to transcriptional dysregulation and tumor progression.

2025-06-02 | PXD064255 | Pride

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