Project description:To understand molecular events of gallbladder cells during nanoplastics exposure, we performed scRNA-seq on gallbladder using 10x Genomics Chromium platform
Project description:The increasing presence of nanoplastics in agricultural soils, particularly polystyrene nanoplastics (PSNPs), poses a novel and underestimated threat to crop productivity and food security. The impact of plastics has recently been investigated in cereals confirming that PSNPs can be absorbed by plants through the roots and subsequently translocated to other plant organs. While extensive research has focused on bread wheat, the effects of PSNPs on durum wheat (Triticum turgidum ssp. durum) remain largely unexplored. In this study, we examined the transcriptomic response to PSNPs exposure in two durum wheat lines: Kronos (wild type) and MRP3, a low-phytate mutant generated via TILLING. The MRP3 line carries deleterious mutation in the Multidrug Resistance-Associated Protein 3 (MRP3) genes, which encodes a vacuolar transporter of phytic acid.
Project description:Plastics are one of the most preoccupying emerging pollutants. Macroplastics released in the environment degrade into microplastics and nanoplastics. Because of their small size, these micro and nano plastic particles can enter the food chain and, in addition to their ecotoxicological effects, contaminate humans with still poorly known biological effects. Plastics being particulate pollutants, they are handled in the human body by scavenger cells such as macrophages, which are important players in the immune system. One of the major issues with plastic contamination of living cells is their extremely variable biodegradability. Many petroleum-based plastics such as polyethylene and polystyrene are very poorly biodegradable, while the degradability of other plastic types such as polyesters is very variable. Moreover, the exposure of humans to plastics is typically a chronic, repeated exposure, and not an acute one as in most of the in vitro toxicology studies devoted to nanoplastics.In this research project, we compare the proteomes of macrophages exposed to repeated doses (8x10µg/ml) of different plastic particles, in order to decipher their responses to these pollutants of concern. We included in this study two poorly biodegradable plastics (polystyrene and poly-ethyleneterephthalate) and two biodegradable plastics (polylactide and polycaprolactone). For comparisons reasons, the data obtained on cells exposed to the same cumulative dose at once (1x80µg/ml) are also included.
Project description:We used LC-MS to detect the protein expression in liver, the main organ of orally injected polystyrene nanoplastics accumulated, so that we can analyze the function change of liver upon polystyrene nanoplastics exposure.
Project description:Gallbladder cancer is a rare but highly malignant cancer. We performed the transcriptional profile sequencing to figure out the potential mechanisms, which might significantly affect gallbladder cancer progression.
Project description:The complexity of transcriptome in human gallbladder has not been clarified quite clearly so far. Here we collect 20 pairs of gallbladder samples.High-throughput RNA sequecing data was generated for each sample.We characterize the linear and circular transcripts.
Project description:Nanoplastics represent an emerging class of global environmental contaminants; however, their potential to interfere with human developmental processes remains largely unexplored. This study investigated the impact of exposure to polystyrene nanoplastics (PS-NP) on the de novo formation, differentiation, and function of human liver organoids derived from induced pluripotent stem cells (hiPSCs), a sophisticated model of early organogenesis.
Project description:Micro and nanoplastics (MNPLs) are contaminants originated mainly from plastic waste degradation that pose potential health risks. Inhalation is a major exposure route, as evidenced by their detection in human lungs, with polyethylene terephthalate (PET) among the most abundant particles in respiratory airways. However, the harmful effects of particle bioaccumulation remain unclear, as chronic effects are understudied. To assess the long-term effects, specifically the carcinogenic effects, BEAS-2B cell line was exposed to PET-NPLs for 30 weeks. Genotoxicity, carcinogenic phenotypic hallmarks, and a panel of genes and pathways associated with cell transformation and lung cancer were examined and compared across three exposure durations. No significant effects were observed after 24 hours or 15 weeks of exposure. However, 30-week exposure led to increased genotoxic damage, anchorage-independent growth, and invasive potential. Transcriptomic analysis showed upregulation of several oncogenes and lung cancer-associated genes at the end of the exposure. Further analysis revealed an increase in differentially expressed genes over time and a temporal gradient of lung cancer-related genes. Altogether, the data suggest PET-NPLs' potential carcinogenicity after extended exposure, highlighting serious long-term health risks of MNPLs. Assessing their carcinogenic risk under chronic, real-life conditions is crucial to address knowledge gaps and eventually develop preventive policies.
Project description:Nanoplastics, as an emerging persistent pollutant of global concern in recent years, have posed a potential threat to human health. However, there is little known about the adverse effects of nanoplastics on the female reproductive system. Here, polystyrene nanoplastics (PS-NPs) with a diameter of 50 nm were selected as representative nanosized plastic particles to investigate the potential effects of subchronic exposure on placenta development in mice.
Project description:We determined the global microRNA expression profiles of primary human gallbladder cells and genetically reprogrammed human gallbladder cells and compared with pancreatic beta cells to ascertain the degree of cellular transdifferentatiation of insulin-producing human gallbladder cells to become beta-like cells. First, we cultured patient-derived gallbladder cells and then we transduced these with beta cell transcription factors to reprogram gallbladder cells to become beta-like cells. We used a pan-islet surface monoclonal antibody to enrich for insulin-producing reprogrammed human gallbladder cells using FACS.