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Screening of differentially expressed genes in LSCC cells knocking down FSCN1

ABSTRACT: Screening of differentially expressed genes in LSCC cells knocking down FSCN1

PROVIDER: PRJNA1073840 | ENA |

REPOSITORIES: ENA

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Screening of differentially expressed genes in LSCC cells knocking down FSCN1

Project description:Laryngeal squamous cell carcinoma (LSCC) is a common malignant tumor with a poor prognosis. Fascin actin-bundling protein 1 (FSCN1) has been reported to play a crucial role in LSCC development and progression, but the underlying molecular mechanisms remain unknown. Here, a whole transcriptome microarray analysis was performed to screen for differentially expressed genes in FSCN1 knockdown cells. We identified 1063 differentially expressed mRNA transcripts. Functional annotation revealed that these differentially expressed genes (DEGs) were involved in multiple biological functions such as transcriptional regulation, response to radiation, focal adhesion, ECM-receptor interaction, steroid biosynthesis, and others. Through co-expression and protein-protein interaction analysis, we linked FSCN1 to novel functions, including defense response to virus and steroid biosynthesis.

2024-02-17 | GSE255143 | GEO

Knocking down Zfp352 delayed mouse embryo development

Project description:Knocking down Zfp352 delayed mouse embryo development

| PRJNA931976 | ENA

Expression data from C2C12 cells after knocking-down lncRNA-MEG3

Project description:The expression level of mRNA after knocking-down lncRNA-MEG3 showed a great significance. We performed microarray and transcriptome profiling in C2C12 cells after transfection lncRNA-MEG3 48 hours later to detail the expression of mRNA after knocking-down lncRNA-MEG3.

2020-12-24 | GSE155070 | GEO

Genome-wide analysis of gene expression regulated by FSCN1 in cervical cancer HeLa cells

Project description:FSCN1 has been reported to be dysregulated in cervical cancers. However, the genome-wide regulated targets of FSCN1 is still unclear in cervical cancers. Here, the gene expression profile of HeLa cells transfected with FSCN1 shRNA (shFSCN1) were compared with cells transfected with empty vector (shCtrl). The results showed that shFSCN1 extensively affects the transcriptional level of 5, 043 genes in HeLa cells. There were more up-regulated genes (3, 870) than down-regulated ones (1, 173) after FSCN1 was knocked down in HeLa cells. GO analysis showed that the up-regulated genes were associated with transcription regulation and DNA binding. The down-regulated genes were enriched in some cancer associated pathway including angiogenesis and cell adhesion. In particular, FSCN1 positilvely regulated ANGPTL4 in HeLa cells. Compared to normal tissue, both FSCN1 and ANGPTL4 showed a higher expression in cervical tumor tissue. Moreover, ANGPTL4 was also positilvely correlated with expression of FSCN1 in cervical tumor tissue of TCGA. In conclusion, our study provide important cues for further study on the regulatory mechanism of FSCN1 in cervical cancer.

2022-01-20 | GSE149450 | GEO

Effect of knocking down HOXB9 in gastric cancer cells

Project description:Effect of knocking down HOXB9 in gastric cancer cells

| PRJNA928345 | ENA

Effect of knocking down PIEZO1 in gastric cancer cells

Project description:Effect of knocking down PIEZO1 in gastric cancer cells

| PRJNA951218 | ENA

Screening of differentially expressed genes in three Plasmodium falciparum parasite lines [expression]

Project description:We performed Agilent chip analysis with synchronized 0-6h ring stage parasites of three P.falciparum lines within their third life cycle, to study the change of expression profiling when knocking down PfSWIB .

2020-02-18 | GSE141404 | GEO

Knocking down CAVIN1 in WPMY-1 normal prostate stromal cells

Project description:Knocking down CAVIN1 in WPMY-1 normal prostate stromal cells

| PRJNA609741 | ENA

Expression data from C2C12 cells after knocking-down lncRNA-MEG3

Project description:Expression data from C2C12 cells after knocking-down lncRNA-MEG3

| PRJNA648375 | ENA

Proteomic Analysis of FSCN1-Interacting Proteins in LSCC cells

Project description:Fascin actin-bundling protein 1 (FSCN1) is an evolutionarily conserved actin-bundling protein that plays a critical role in cell migration, motility, adhesion and cellular interactions. Although multiple clinical studies have implicated the expression of FSCN1 in laryngeal squamous cell carcinoma (LSCC) progression, the precise mechanism of FSCN1 in the process has not been clearly elucidated. To define FSCN1 function, we characterized FSCN1-interacting proteins in LSCC cells by immunoprecipitation followed by LC-MS/MS. After data filtering, 119 proteins with expression in both the Hep-2 and TU-177 cell samples were identified as FSCN1-interacting partners. With in-depth bioinformatics analysis, we linked FSCN1 to critical cellular processes including cell adhesion, glycolysis/gluconeogenesis, regulation of protein ubiquitination, ribosomal RNA processing and small molecule metabolism. We discuss the interactions between FSCN1 and some of the newly validated partners. The identification of these potential partners of FSCN1 expands our knowledge of the FSCN1 interactome and provides a valuable resource for understanding the functions of this protein in LSCC progression.

2019-09-09 | PXD013072 | Pride

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