Project description:Wild-type and two different Parp3-/- clones of C2C12 cells were induced to differentiate for 72h. Microarray was performed to compare the transcriptomic profile of the two Parp3-deficient clones versus the wild-type control at T72h post-differentiation.
Project description:Heading date1(Hd1) is a critical regulator controlling rice flowering time, which promotes flowering under short-day (SD) conditions and represses flowering under long-day (LD) conditions. In our previous study (Luan et al., 2009), we identified a rice mutant, hd1-3, in which the Hd1 gene was deficient due to several insertions/deletions in the coding region. To search for downstream genes regulated by Hd1, we performed microarray analysis of hd1-3 mutant and the wild-type Zhonghua11 under both SD and LD conditions. According to the microarray results, SDG712 gene was significantly downregulated in the hd1-3 mutant, indicating that SDG712 gene may acts downstream of Hd1, and may functions in rice flowering time regulation.
Project description:C2C12 is a myoblast cell line usually used for study of muscle differentiation. Myod1 is a pro-differentiation factor for myogenesis. We found that CRISPR/Cas9-mediated Myod1 gene knockout in C2C12 cells led to the loss of myoblast identity and gain of neural properties.
Project description:camta3-1, camta3-2 and WT microarray experiments, statistical and bioinformatics analysis Keywords: expression analysis; mutant versus wild type comparison