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Alteration of BRN3A expression influences the expression of genes involved in the regulation of important biological processes in the HPV induced cervical Cancer

ABSTRACT: Alteration of BRN3A expression influences the expression of genes involved in the regulation of important biological processes in the HPV induced cervical Cancer

PROVIDER: PRJNA1074375 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27906826_1.fastq.gz	Fastqsanger.gz
	SRR27906826_2.fastq.gz	Fastqsanger.gz
	SRR27906827_1.fastq.gz	Fastqsanger.gz
	SRR27906827_2.fastq.gz	Fastqsanger.gz
	SRR27906828_1.fastq.gz	Fastqsanger.gz

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Alteration of BRN3A expression influences the expression of genes involved in the regulation of important biological processes in the HPV induced cervical Cancer

Project description:Cervical cancer is the fourth most common cancer worldwide in females. This occurs primarily due to the infection of high-risk human papillomavirus (HPV) although in advanced stages it requires support from host cellular factors. BRN3A is one such host cellular factor that upregulates tumorigenic high-risk HPV expression. The expression of BRN3A in cervical cancer remains high where it enhances tumorigenicity. The effect of BRN3A on HPV-mediated cervical cancer and the underlying mechanism remains obscure. Thus, we have investigated the overall effect of BRN3A on genes associated with cancer-related different biological processes. In approach, we have altered the expression of BRN3A through overexpression and knockdown in cervical cancer cells following transcriptome profiling through next-generation RNA-sequencing. This study revealed a substantial change in the expression of several genes associated with cancer-promoting biological processes including viral processes, immune response, cell-death, cell-proliferation, and different signaling pathways, etc. Additionally, promoter analysis through in silico mode revealed that a total of 32.7% of genes possess BRN3A binding sites at their promoters. Physical interaction of BRN3A with IFITM1, OAS3, ISG15, BCL2L1, and HSP90AB1 genes was also confirmed. Thus, the present study identified molecular targets of BRN3A and provided new insight into the pathogenesis of cervical cancer. According to our knowledge, this is the first report on the effect on eukaryotic transcriptomes after over-expression and knocking down BRN3A.

2025-02-06 | GSE255288 | GEO

HPV genome expression at invasive cervical carcinomas infected by multiple HPV genotypes

Project description:Multiple HPV genotypes infection is frequently detected in HPV+ cervical lesions, however it is not well stablished how is the different viral interaction during the carcinogenic process. Here we carried out a comprehensive study to characterize the multiple HPV genome expression and integration by RNA-Seq analysis in 19 invasive cervical carcinomas with HPV coinfections. Analysis of tumoral DNA by a hybridization kit indicated multi-infection ranging from 2 to 6 different HPV genotypes, without a preferential species coinfection. The expression analysis showed that a single HPV genotype preferentially expressed its genome, might indicating a competition between the infecting virus. Finally, the search for HPV/human chimeric transcripts indicated integration from just one HPV in almost all samples, corroborating the expression findings.

2021-07-28 | GSE144293 | GEO

Gene Expression Profiles of HPV-Positive and -Negative Head/Neck and Cervical Cancers

Project description:Human papillomaviruses (HPVs) are associated with nearly all cervical cancers (CCs), 20-30% of head and neck cancers (HNCs), and other cancers. Because HNCs also arise in HPV-negative patients, this type of cancer provides unique opportunities to define similarities and differences of HPV-positive versus HPV-negative cancers arising in the same tissue. Here, we describe genome-wide expression profiling of 84 HNCs, CCs and site-matched normal epithelial samples in which we used laser capture microdissection to enrich samples for tumor-derived versus normal epithelial cells. This analysis revealed that HPV+HNCs and CCs differed in their patterns of gene expression yet shared many changes compared to HPV-HNCs. Some of these shared changes were predicted, but many others were not. Notably, HPV+HNCs and CCs were found to be upregulated in their expression of a distinct and larger subset of cell cycle genes than observed in HPV-HNC. Moreover, HPV+ cancers over-expressed testis-specific genes that are normally expressed only in meiotic cells. Many, though not all, of the hallmark differences between HPV+HNC and HPV-HNC were a direct consequence of HPV and in particular the viral E6 and E7 oncogenes. This included a novel association of HPV oncogenes with testes specific gene expression. These findings in primary human tumors provide novel biomarkers for early detection of HPV+ and HPV- cancers, and emphasize the potential value of targeting E6 and E7 function, alone or combined with radiation and/or traditional chemotherapy, in the treatment of HPV+ cancers. Keywords: Gene expression ptofiles of primary cancers

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HPV genome expression at invasive cervical carcinomas infected by multiple HPV genotypes

Project description:HPV genome expression at invasive cervical carcinomas infected by multiple HPV genotypes

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Project description:The study examined the infection state of HPV in the Uyghur population with cervical cancer, followed by genotyping to determine the variation in the types of HPV. Using microRNA microarray technology, differential gene expression between HPV-infected cervical cancer and uninfected normal cervical tissues was determined. The microarray results were verified by qRT-PCR using 20 sets of HPV-infected cervical cancer and uninfected cervical tissues.

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Protecting Young Special Risk Females From Cervical Cancer Through Human Papilloma Virus (HPV) Vaccination

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