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Overexpressing the ClpC AAA+ Unfoldase Prematurely Triggers Secondary Differentiation in Chlamydia trachomatis

ABSTRACT: Overexpressing the ClpC AAA+ Unfoldase Prematurely Triggers Secondary Differentiation in Chlamydia trachomatis

PROVIDER: PRJNA1075127 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR27931661_1.fastq.gz	Fastqsanger.gz
	SRR27931661_2.fastq.gz	Fastqsanger.gz
	SRR27931662_1.fastq.gz	Fastqsanger.gz
	SRR27931662_2.fastq.gz	Fastqsanger.gz
	SRR27931663_1.fastq.gz	Fastqsanger.gz

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Overexpressing the ClpC AAA+ Unfoldase Prematurely Triggers Secondary Differentiation in Chlamydia trachomatis

Project description:Chlamydia is an obligate intracellular bacteria that undergoes a complex biphasic developmental cycle, alternating between the smaller infectious non-dividing elementary body (EB) and the larger non-infectious but dividing reticulate body (RB). Due to the differences between these functionally and morphologically distinct forms, we hypothesize protein degradation is essential to chlamydial differentiation. The bacterial Clp system, consisting of an ATPase unfoldase (e.g., ClpX or ClpC) and a proteolytic component (e.g., ClpP), is critical for the physiology of bacteria through its recognition, and usually degradation, of specific substrates. We observed by transmission electron microscopy that overexpression of wild-type ClpC, but not an ATPase mutant isoform, in Chlamydia increased glycogen accumulation within the vacuolar niche of the bacteria earlier in the developmental cycle than typically observed. This suggested ClpC activity may increase expression of EB-associated genes. Consistent with this, targeted RT-qPCR analyses demonstrated a significant increase in several EB-associated gene transcripts earlier in development. These effects were not observed with overexpression of the ATPase mutant of ClpC, providing strong evidence that the activity of ClpC drives secondary differentiation. By analyzing the global transcriptional response to ClpC overexpression using RNA sequencing, we observed a global shift to earlier expression of canonical late developmental cycle genes and other EB-associated genes. Finally, we directly linked overexpression of ClpC with earlier production of EBs. Conversely, disrupting normal ClpC function with an ATPase mutant caused a delay in secondary differentiation. Overall, these findings provide the first mechanistic insight for initiation of secondary differentiation in Chlamydia.

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Project description:Chlamydia trachomatis is an important human pathogen that replicates inside the infected host cell in a unique vacuole, the inclusion. The formation of this intracellular bacterial niche is essential for productive Chlamydia infections. Despite its importance for Chlamydia biology, a holistic view on the protein composition of the inclusion, including its membrane, is currently missing. Here we describe a newly established method to purify inclusions from C. trachomatis infected epithelial cells and the analysis of the host cell-derived proteome by a combination of label free and stable isotope labeling -based quantitative proteomics. Computational analysis of the proteome data indicated that the inclusion is a complex intracellular trafficking platform that interacts with host cells' antero- and retrograde trafficking pathways. Furthermore, the inclusion is highly enriched for sorting nexins of the SNX-BAR retromer, a complex essential for retrograde trafficking. Functional studies showed that in particular SNX5 controls the C. trachomatis infection and that retrograde trafficking is essential for infectious progeny formation. In summary, our findings suggest that the inclusion of C. trachomatis is well embedded in the hosts' endomembrane system and hijacks retrograde trafficking pathways for effective infection.

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