Project description:Gene expression patterns were assessed in normal human bronchial epithelial (NHBE) cells exposed to cigarette smoke (CS) from a typical "full flavor" American brand of cigarettes in order to develop a better understanding of the genomic impact of tobacco exposure, which can ultimately define biomarkers that discriminate tobacco-related effects and outcomes in a clinical setting. NHBE cells were treated with CS for 15 minutes and alterations to the transcriptome assessed at 1,2,4 and 24 hours post-CS-exposure using high-density oligonucleotide microarrays. Keywords: Time course

2008-03-05 | GSE10718 | GEO

Transcription profiling of human NHBE cells exposed TO NORMAL human bronchial epithelial whole cigarette smoke FROM light AND standard brands

Project description:Gene expression patterns were assessed in normal human bronchial epithelial (NHBE) cells exposed to cigarette smoke from a reference cigarette (2R4F, University of Kentucky) and a typical American brand of "light" cigarettes ("Lights") in order to develop a better understanding of the genomic impact of tobacco exposure, which can ultimately define biomarkers that discriminate tobacco-related effects and outcomes in a clinical setting. NHBE cells were treated with whole cigarette smoke for 15 minutes and alterations to the transcriptome assessed at 2, 4, 8 and 24 hours post-exposure using high-density oligonucleotide microarrays. Experiment Overall Design: 4 replicate Petri dishes of cells were exposed in a custom-built smoke exposure chamber. Cigarettes were smoked as per FTC protocols, and the smoke diluted such that the cells were at least 50% viable as compared to mock (air)-exposed controls after 24h. RNA from each replicate dish was analyzed using a separate array. Four replicates of an incubator (untreated with either smoke or air) are included also.

2008-04-06 | E-GEOD-10700 | biostudies-arrayexpress

E-MEXP-1512

Project description:Transcription profiling of human bronchial epithelial cells following exposure to whole cigarette smoke

2025-01-22 | E-MEXP-1512 | ExpressionAtlas

NHBE exposed to cigarette smoke

Project description:normal human bronchial epithelial cultures from two cultures in parallel exposed to cigarette smoke (CS) or air (mock) at timepoints 4 hours and 24 hours. Keywords = cigarette smoke Keywords = microarray Keywords = bronchial cell Keywords = tobacco Keywords: time-course

2005-02-23 | GSE2302 | GEO

E-GEOD-18344

Project description:Transcription profiling by array of Nrf2 knockout mice exposed to cigarette smoke

2015-09-15 | E-GEOD-18344 | ExpressionAtlas

Temporal transcriptional response of endothelial cells to aqueous cigarette smoke extract exposure

Project description:Endothelial cells (HUVEC) were exposed to cigarette smoke extract for 3, 7, and 24 hours. Total RNA was isolated and used for hybridisation to dual channel oligonucleotide genome-wide microarrays using untreated HUVEC as a control. The objective was to find out what genes are regulated by exposure to cigarette smoke extract, and the time dependency of their regulation Keywords: time course, stress response

2007-01-26 | GSE6854 | GEO

Transcription profiling of human normal human bronchial epithelial (NHBE) cells exposed to whole cigarette smoke over a time series to study the impact of tobacco exposure

2008-04-06 | E-GEOD-10718 | biostudies-arrayexpress