Project description:We performed an analysis of transcriptomic responses to auxin within four distinct tissues of the Arabidopsis thaliana root. This high-resolution dataset shows how different cell types are predisposed to react to auxin with discrete transcriptional responses. The sensitivity provided by the analysis lies in the ability to detect cell-type specific responses diluted in organ-level analyses. This dataset provides a novel resource to examine how auxin, a widespread signal in plant development, influences differentiation and patterning in the plant through tissue-specific transcriptional regulation.
Project description:Woody biomass is a critical renewable resource for sustainable energy and material production, yet strategies to increase its yield while simultaneously improving quality remain limited. Here, we demonstrate that xylem-specific overexpression of Arabidopsis thaliana LATERAL ORGAN BOUNDARIES DOMAIN (LBD) transcription factors markedly enhances stem secondary growth and alters wood composition in hybrid aspen (Populus tremula × Populus tremuloides Michx., clone T89). Using the LMX5 promoter to drive targeted expression of A. thaliana LBD4 and LBD11 in the developing xylem, we observed substantial stimulation of secondary xylem formation. Histological analyses revealed distinct roles for each gene: LBD4 primarily promoted xylem development through increased cell expansion, whereas LBD11 enhanced both cell proliferation and expansion. This dataset was used to analyze the LBD overexpression lines
Project description:The objective of the study is to profile histone H3 lysine nine di-methylation (H3K9me2) in Arabidopsis thaliana and to correlate it with DNA methylation.
