Project description:We used adult male Sprague-Dawley rats (280-329 g body weight). Controls were naïve rats. Ischemic rats were subjected to 1-hour occlusion of the right middle cerebral artery and 16h reperfusion. The goal was to identify the changes in the gene expression profile of PV after brain ischemia.
Project description:Analysis of embronic day 30.5 (E30.5) fetal rabbit left lung after creation of diapragmatic hernia (DH) at E25 and tracheal occlusion (TO) at E27. The fetuses of timed pregnant New Zealand rabbits were subjected to creation of a left diaphragmatic hernia at E25 with or without tracheal occlusion at E27. At E30.5, the fetuses were sacrificed with collection of the lateral aspect of the left upper lobe for NextGen mRNA sequencing.
Project description:To investigate time-dependent changes in the epigenetic chromatin state in microglia after ischemic stroke, we performed assay for transposase-accessible chromatin with sequencing (ATAC-seq) of microglia from mouse brains at multiple time points after middle cerebral artery occlusion (MCAO).
Project description:<p>Rapid, deep, and sustained systemic ketosis was achieved using an orally administered ketone ester (KE: 3.0 mg/g body weight of (R)-3-hydroxybutyl (R)-3-hydroxybutyrate; DeltaG® Ketone Ester, TΔS® Ltd, Oxfordshire, UK) compared with control (0.9% NaCl) prior to permanent middle cerebral artery occlusion (pMCAO; n=5 mice per group). The severity of cerebral ischemia was assessed in vivo by MRI at 60 minutes after arterial occlusion. To investigate the underlying mechanisms, ex vivo metabolomic analysis of brain tissue was performed. The brain was separated into the ischemic hemisphere and the contralateral control hemisphere.</p>