Project description:Small RNA-seq of exosomes derived from epithelial cells of skin tissue on the side of the body of Inner Mongolia cashmere goats at 55 days of fetal development
Project description:Surveillance of tick-associated viruses may contribute to our understanding of viral diversity and evolution. We identified a novel viral sequence from a sample derived from a human-biting tick, Amblyomma testudinarium, in Japan. The viral sequence that we found was 4621 bp in length with 54% GC content. Two major open reading frames were predicted to encode a putative coat protein (CP) and an RNA-dependent RNA polymerase (RdRp), respectively. Two ORFs overlapped at the tetranucleotides, AUGA. The C-terminal region of putative CP was enriched in alanine, glycine, and proline residues. All these features are similar to those commonly observed in victoriviruses. Phylogenetic analyses based on the amino acid sequences of the putative CP and RdRp showed that the virus belongs to the genus Victorivirus of the family Pseudototiviridae. Based on these, we designated this putative virus as Amblyomma testudinarium-associated victorivirus 1 (ATaVV1). These findings expand current knowledge of hidden diversity of virome associated with tick-related samples.