Project description:The zona pellucida plays a crucial role in the process of fertilization to early embryonic development, including cellular arrangement and communication between blastomeres. However, little is known regarding the role of the zona pellucida in pre- and post-implantation embryonic development associated with gene expression. We investigated the effects of the zona pellucida on the development and gene expression of pre- and post-implantation embryos. After the zona pellucida was removed at the 2-cell stage, compaction occurred earlier. In addition, the expression of differentiation-related genes in the inner cell mass (ICM) (Oct4, Sox21) and trophectoderm (TE) (Cdx2, Eomes, and Tfap2c) was significantly altered in both zona free (ZF) morula and blastocyst compared to zona intact embryos. After embryo transfer, the implantation rate and number of live fetuses were lower in ZF embryos than in control embryos, whereas the fetal weight at birth was not different. However, placental weight was significantly increased in ZF embryos. RNA-seq analysis of the placenta showed that a total of 473 differentially expressed genes that significantly influenced the biological process. The present study suggests that zona pellucida removal at the 2-cell stage not only disturbs the expression pattern of ICM/TE-related genes, but affects the post-implantation development of mouse embryos. Overall, this study provides a deeper insight into the role of the zona pellucida during early mouse embryonic development and the viability of post-implantation development. It also provides a valuable basis for future research related to the production of ZF embryos in mammals and the developmental properties of blastomeres.

2022-03-31 | GSE199670 | GEO

Haloarcula pellucida strain:CECT 7537

Project description:Haloarcula pellucida strain:CECT 7537 Genome sequencing and assembly

| PRJNA504762 | ENA

Formation mechanism of agar-like zona pellucida and its adverse effects on human oocytes

Project description:Agar-like zona pellucida (ZP) is a type of ZP abnormality that adversely affects oocyte maturation, fertilization, and developmental potential. However, the mechanisms underlying its formation and its detrimental effects on oocytes remain elusive. Through comprehensive analyses including whole-exome sequencing, transcriptome sequencing, Q-PCR, scanning electron microscopy, and transmission electron microscopy, our study found that the c.2785G>C mutation in ATRX may represent a genetic factor contributing to the agar-like ZP formation. Moreover, while mutations in ZP1-4 genes were not essential for agar-like ZP formation per se, an increased proportion of ZP1 protein among the four ZP proteins contributed to this phenomenon. Additionally, up-regulation of PMP22 may also be implicated in agar-like ZP formation and exert deleterious effects on oocyte development. Our findings enhance our understanding of agar-like ZP and provide a theoretical foundation for its treatment in assisted reproduction. Furthermore, our study demonstrates potential applications in contraception as the agar-like ZP effectively inhibits conventional fertilization of oocytes.

2026-04-25 | GSE239936 | GEO

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