Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1079549 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:We used wheat as rotational crop to assess the influence of continuous cropping on microbiome in Pinellia ternata rhizosphere and the remediation of rotational cropping to the impacted microbiota. Illumina high-throughput sequencing technology was utilized for this method to explore the rhizosphere microbial structure and diversity based on continuous and rotational cropping.
2016-12-10 | GSE91083 | GEO
Project description:This project is designed for whole transcriptome sequencing of bacteria isolated from Rhizosphere of Wheat Plant, which has its impact on overall plant growth.
2024-02-28 | E-MTAB-11516 | biostudies-arrayexpress
Project description:Phosphate (P) fertilization impacts many rhizosphere processes, driving plant P use efficiency. However, less is known about the induced molecular and physiological root-rhizosphere traits in responses to polyphosphates (PolyP), particularly root transcriptome and belowground functional traits responsible for P acquisition. The present study aims to investigate physiological and transcriptomic belowground mechanisms explaining the enhanced durum wheat P acquisition under PolyP (PolyB and PolyC) supply. Root molecular traits were differentially expressed in response to PolyP, where PolyB induced upregulation of OGDH, MDH, and ENO, PAP21 and downregulation of PFK, and LDH genes. The modulation of gene expression can presumably explain the PolyP-induced changes in rhizosphere (root, rhizosphere soil, soil solution) acidification (pH decreased from 8 to 6.3) and acid phosphatase activities, which were concomitant with enhanced rhizosphere soil P availability and shoot Pi content (145% and 36% compared to OrthoP, respectively) along with changes in morphological and transcriptomic root (particularly, the upregulation of AUX1 and ABA transporter genes) traits. These findings provide novel insights that P acquisition from polyphosphates involves the coordinated regulation of genes governing root-rhizosphere processes and root development, ultimately enhancing wheat P acquisition.
2025-05-31 | GSE277488 | GEO
Project description:<p>Background</p><p>Wheat crown rot (WCR) caused by Fusarium spp. lacks durable, sustainable control. Engineering the rhizosphere with defined synthetic microbial communities (SynComs) offers a route to combined disease suppression and growth promotion. We aimed to build a cross-kingdom SynCom and evaluate its impacts on plant performance and the soil–microbiome system.</p><p>Results</p><p>We assembled a two-member SynCom comprising an antagonistic fungus (Trichoderma harzianum) and a growth-promoting bacterium (Bacillus rugosus). In greenhouse trials, SynCom inoculation reduced WCR severity by ~71% and improved vigor, more than doubling shoot and root biomass and increasing grain weight by ~13% versus non-inoculated controls. SynCom-treated plants maintained higher chlorophyll and antioxidant enzyme activities under pathogen challenge, with reduced oxidative stress markers relative to pathogen-only plants. Amplicon sequencing showed increased rhizosphere microbial diversity, enrichment of beneficial taxa (e.g., Mortierella), and suppression of Fusarium. SynCom also enhanced soil enzyme activities and nutrient availability and promoted accumulation of defense-related metabolites in the rhizosphere.</p><p>Conclusions</p><p>A tailored cross-kingdom SynCom establishes a disease-suppressive, growth-promoting soil environment that mitigates wheat crown rot while improving yield components. These findings support microbiome engineering as a practical, sustainable strategy for wheat production and warrant field-scale validation and formulation development.</p>
2025-09-06 | MTBLS12945 | MetaboLights
Project description:<p>Wheat is a major staple crop grown across the globe. Fusarium crown rot (FCR) of wheat, caused by Fusarium pseudograminearum, is a destructive soil-borne disease that lacks effective sustainable control measures. Here, we assembled a cross-kingdom synthetic microbial community (SMC) comprising Trichoderma harzianum T19, Bacillus subtilis BS-Z15, and four other Bacillus strains, and evaluated its biocontrol efficacy against FCR under non-sterile soil conditions. The SMC treatment significantly suppressed FCR, reducing the disease severity index by approximately 70%. Wheat growth and yield were simultaneously enhanced: SMC inoculation nearly doubled plant biomass (with fresh and dry weights ~100% higher) and increased thousand-kernel weight by ~14% compared to the controls. In the rhizosphere, SMC improved soil health by elevating soil organic matter and nitrogen levels by over 50%, while mitigating pathogen-induced nutrient imbalances (excess available P and K) and boosting nutrient-cycling enzyme activities. Amplicon sequencing revealed that SMC suppressed pathogenic Fusarium in the rhizosphere and enriched beneficial microbes, including antagonistic fungi (Trichoderma, Chaetomium) and plant growth-promoting bacteria (Pseudomonas, Paenibacillus). Co-occurrence network analysis showed that SMC treatment restructured the rhizosphere microbial network with higher connectivity, stability, and a prevalence of positive cooperative interactions under F. pseudograminearum stress. Defense-related metabolites, such as epi-jasmonic acid, allantoin, Nβ-acetyltryptamine, and dihydrodaidzein, accumulated to higher levels with SMC, consistent with KEGG enrichment in pathways related to amino acid biosynthesis, carbon metabolism, signal transduction, and plant defense. These findings demonstrate that the cross-kingdom SMC modulates soil nutrients, microbial community structure, and rhizosphere metabolites to synergistically promote wheat growth and enhance resistance to FCR.</p>
2025-09-06 | MTBLS12946 | MetaboLights