Project description:Propylbenzene (PBZ) is an indispensable commodity in chemical industry, microalgae have the ability to propagate in open water by utilizing nutrients, thus contributing to the global nutrient cycle (C, N, and P). We set out to elucidate the metabolic mechanisms of n-PBA using Rhinomonas reticulata S6A, isolated from Daya Bay in our previous study (Du et al., 2023). By utilising 4D-data-independent acquisition (4D-DIA) for proteomic analysis. The outcomes of this study will contribute to the development of marine microalgae-based remediation technologies, offering a viable alternative for the treatment of PBZ contamination.
Project description:Identification of miRNAs in citrus reticulata exosomes;Identification of potential target genes of exosomal miRNAs in penicillium italicum; Comparison of differentially expressed genes between citrus exosome-treatedpenicillium italicum and wild type
Project description:Ovule developmental arrest is one of the early phenotypes of seed abortion. However, the molecular mechanism underlying ovule developmental arrest of citrus is still unclear. In this study, laser capture microdissection (LCM) was used to accurately divide the ovules of seedless Ponkan ‘Huagan NO.4’ (Citrus reticulata) (MT) and seeded Ponkan ‘Egan NO.1’ (Citrus reticulata) (WT) into nucellus and integument tissues. The captured tissues were used for subsequent RNA-seq. Moreover, single-molecule real-time (SMRT) sequencing was used to generate full-length transcripts of WT, which were used as reference transcripts for RNA-seq. These data can be utilized to analyse the causes of citrus seedlessness formation and the molecular regulatory network in the process of ovule abortion.
Project description:Seed developmental arrest is one of the early phenotypes of seed abortion. However, the molecular mechanism underlying seed developmental arrest of citrus is still unclear. In this study, laser capture microdissection (LCM) was used to accurately divide the seeds of seedless Ponkan ‘Huagan No.4’ (Citrus reticulata) (HG) and seeded Ponkan ‘Egan No.1’ (Citrus reticulata) (EG) into nucellus and integument/seed coat tissues. The captured tissues were used for subsequent RNA-seq. Moreover, single-molecule real-time (SMRT) sequencing was used to generate full-length transcripts of EG, which were used as reference transcripts for RNA-seq. These data can be utilized to analyse the causes of citrus seedlessness formation and the molecular regulatory network in the process of seed abortion.