Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of GSK3 inhibition on the transcriptional signature of human monocytes

ABSTRACT: Effect of GSK3 inhibition on the transcriptional signature of human monocytes

PROVIDER: PRJNA1080033 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR28083535_1.fastq.gz	Fastqsanger.gz
	SRR28083535_2.fastq.gz	Fastqsanger.gz
	SRR28083536_1.fastq.gz	Fastqsanger.gz
	SRR28083536_2.fastq.gz	Fastqsanger.gz
	SRR28083537_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 18

Similar Datasets

Effect of GSK3 inhibition on the transcriptional signature of human monocytes

Project description:We explored the molecular impact of blocking GSK3 on the gene expression profile in human monocytes. GSK3 inhibition by CHIR-99021 redirected monocytes towards acquiring an anti-inflammatory gene profile similar to M-CSF-primed monocyte-derived macrophages.

2025-04-16 | GSE256538 | GEO

Effect of GSK3 inhibition on the transcriptional signature of human alveolar macrophages

Project description:Effect of GSK3 inhibition on the transcriptional signature of human alveolar macrophages

| PRJNA1091920 | ENA

Effect of GSK3 inhibition on the transcriptional signature of human alveolar macrophages

Project description:The remarkable plasticity of macrophages is tied to extracelular cues and their subsequently signaling pathways. The transcriptional factor MAFB is critical to prompt the aquisition of an anti-inflammatory phenotype in macrophages. As MAFB activity and stability is highly regulated by GSK3, we explored the molecular impact of blocking GSK3 on the gene expression profile of human alveolar macrophages (AMØ), whose development and gene expression profile is GM-CSF-dependent. GSK3 inhibition skewed the transcriptional profile of AMØ towards an anti-inflammatory phenotype.

2025-04-16 | GSE262463 | GEO

Effect of GSK3 inhibition on the transcriptional signature of human GM-CSF-dependent monocyte-derived macrophages

Project description:The functional versatility of macrophages is intrincately tied to factors such as their ontogeny and the specific tissue and extracellular environment. Monocyte-derived macrophages are oppositely instructed by M-CSF or GM-CSF. GM-CSF drives monocyte-derived macrophages towards heightened pro-inflammatory activity and the acquisition of the lung alveolar macrophage phenotype and gene profile whereas M-CSF gives rise to anti-inflammatory, pro-resolving, and immunosuppressive monocyte-derived macrophages. We explored the molecular impact of blocking GSK3 on the gene expression profile in GM-CSF-primed human monocyte derived macrophages. GSK3 inhibition skewed the transcriptional profile of GM-MØ towards an anti-inflammatory phenotype.

2025-04-16 | GSE256208 | GEO

Effect of GSK3 inhibition on the transcriptional signature of human GM-CSF-dependent monocyte-derived macrophages

Project description:Effect of GSK3 inhibition on the transcriptional signature of human GM-CSF-dependent monocyte-derived macrophages

| PRJNA1078416 | ENA

Polyubiquitination analysis upon GSK3 inhibition in HeLa cell extracts

Project description:HeLa cell extracts with or without GSK3 enzyme inhibition were assayed using protein microarrays in order to detect GSK3-dependent changes in protein polyubiquitination.

2014-05-15 | GSE50629 | GEO

Polyubiquitination analysis upon GSK3 inhibition in HeLa cell extracts

Project description:HeLa cell extracts with or without GSK3 enzyme inhibition were assayed using protein microarrays in order to detect GSK3-dependent changes in protein polyubiquitination. HeLa lysates in triplicates were supplemented with ubiquitin and incubated on protein microarrays (ProtoArray 5.0; Invitrogen) in the presence or absence of the GSK3 inhibitor SB-216763. Polyubiquitination of the arrayed proteins was detected using specific antibodies. ProtoArray 5.0 contains over 9,000 full-length human proteins purified and arrayed in duplicate under native conditions to maximize functionality.

2014-05-15 | E-GEOD-50629 | biostudies-arrayexpress

RNA-Seq for transcriptome analysis of germinal center B cells upon GSK3 inhibition

Project description:To compare transcriptional changes of germinal center B cells upon GSK3 inhibition, induced GC B cells were treated with GSK3 inhibitor, CHIR99021. 407 differentially expressing genes were identified

2020-11-22 | GSE144361 | GEO

GSK3 inhibition Induces Glioma Cell Death through c-MYC, NF-kB and Glucose Regulation

Project description:We identified GSK3 as a regulator of GBM cell survival using microarray analysis, small molecule and genetic inhibitors of GSK3 activity. Various molecular and genetic approaches were then employed to dissect out the molecular mechanisms responsible for GSK3 inhibition-induced cytotoxicity. Keywords: time course

2009-01-01 | GSE11285 | GEO

Single cell RNAseq of human cortical organoids investigating the effect of chronic treatment with GSK3 inhibitor CHIR99021

Project description:Here we used human cortical brain organoids to probe the longitudinal impact of GSK3 inhibition through multiple developmental stages. Chronic GSK3 inhibition increased the proliferation of neural progenitors and caused massive derangement of cortical tissue architecture. Cortical organoids were differentiated as previously described (Paşca et al., 2015, doi: 10.1038/nmeth.3415.). Chronic GSK3 inhibition was performed by adding CHIR99021 (Merck SML1046) to the medium at day 0 (1 microM) and kept throughout the differentiation process until reaching the respective collection timepoints (day 50, day 100).

2020-06-09 | E-MTAB-8337 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data