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ABSTRACT: Transcriptome and Metabolite Conjoint Analysis Reveals the Seed Dormancy Release Process of Perilla

PROVIDER: PRJNA1080333 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28092101_1.fastq.gz	Fastqsanger.gz
	SRR28092101_2.fastq.gz	Fastqsanger.gz
	SRR28092102_1.fastq.gz	Fastqsanger.gz
	SRR28092102_2.fastq.gz	Fastqsanger.gz
	SRR28092103_1.fastq.gz	Fastqsanger.gz

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affy_dormance_mrnaoxidized_sunflower-Molecular mechanisms of seed dormancy release: transcriptome evolution and ROS signalling during sunflower seed after-ripening.

Project description:affy_dormance_sunflower - affy_dormance_mrnaoxidized_sunflower - We focus our research on the release of embryonic dormancy in sunflower seeds and more precisely on the molecular mechanisms controlling seed dormancy release during dry after-ripening. Our data show an increase of ROS during dormancy release associated with an increase of stored mRNA oxidation during after-ripening as shown with determination of the RNA oxidation marker : 8-hydroxyguanosine (8OHG) To assess to role of mRNA oxidation in this process, we isolated 8OHG-containing mRNA to identify oxidized transcript using microarray analysis.-The samples compared are the following: 1) 8OHG containing mRNA isolated from total RNA extracted from a population of 100 embryonic axis of dry dormant seed (Dox) ie. Seed which have been frozen at -30°C after harvest to keep dormancy 2) 8OHG containing mRNA isolated from total RNA extracted from a population of 50 embryonic axis from dry non dormant ie. Seeds which have been after-ripened (stored) at 70% RH during 2 month (NDox) Replicates correspond to different batches of seeds from the same harvest, which have been treated (after ripened) independently and from which the 8OHG containing fraction have been isolated independently. 4 arrays - SUNFLOWER; dormancy

2011-07-19 | E-GEOD-23048 | biostudies-arrayexpress

affy_dormance_totalrna_sunflower-Molecular mechanisms of seed dormancy release: transcriptome evolution and ROS signalling during sunflower seed after-ripening.

Project description:affy_dormance_sunflower - affy_dormance_totalrna_sunflower - We focus our research on the release of embryonic dormancy in sunflower seeds and more precisely on the molecular mechanisms controlling seed dormancy release during dry after-ripening. Our data show an increase of ROS during dormancy release associated with an increase of stored mRNA oxidation during after-ripening as shown with determination of the RNA oxidation marker : 8-hydroxyguanosine (8OHG) To assess to role of mRNA oxidation in this process, we isolated 8OHG-containing mRNA to identify oxidized transcript using microarray analysis.-The samples compared are the following: 1) Population of 50 embryonic axis from dry dormant seed (D) ie. Seed which have been frozen at -30°C after harvest to keep dormancy 2) Population of 50 embryonic axis from dry non dormant ie. Seeds which have been after-ripened (stored) at 70% RH during 2 month. 3) Embryonic axis from dry dormant seed but after ripened at very low relative humidity (2% RH) (control of storage: S). Replicates correspond to different batches of seeds from the same harvest which have been treated (after ripened) independently. - We want to compare D vs ND; D vs S and ND vs S. 6 arrays - SUNFLOWER; dormant vs non dormant seed embryo

2011-07-19 | E-GEOD-23046 | biostudies-arrayexpress

Project description:Transcriptomic Study about Seed Dormancy release process in Epimedium wushanese

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affy_dormance_totalrna_sunflower-Molecular mechanisms of seed dormancy release: transcriptome evolution and ROS signalling during sunflower seed after-ripening.

2011-07-20 | GSE23046 | GEO

Comparative Transcriptome Analysis of callery pear (Pyrus calleryana Decne) seeds at three different stages of cold stratification

Project description:We carried out the transcriptome analysis to explore expression profiles differences and identify the key genes involved in pear seed dormancy release, by comparing callery pear (Pyrus calleryana Decne) seeds at three different stages of cold stratification

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affy_dormance_mrnaoxidized_sunflower-Molecular mechanisms of seed dormancy release: transcriptome evolution and ROS signalling during sunflower seed after-ripening.

2011-07-20 | GSE23048 | GEO

Abscisic acid regulates plant growth and flowering through SINGLE FLOWER TRUSS pathway in tomato.

Project description:Abscisic acid (ABA) regulates seed and bud dormancy. We show by forward and reverse genetic analysis that the tomato transcription factor SlZFP2 is required for release of bud and seed dormancy through negative regulation of ABA biosynthesis. We also demonstrated that ABA promotes growth and represses flowering in tomato both through transcriptional control on the florigen-encoding gene SINGLE FLOWER TRUSS (SFT) in tomato. To gain further insight on transcriptome changes by overexpresion of HA-SlZFP2, we sequenced two lines of p35S:HA-SlZFP2 in LA1589 background and their nontransgenic siblings on Illumina Hiseq2000 platform.

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Effect of myb107 mutation and temperature on seedcoat and endosperm gene expressions during mature green stage

Project description:Seed dormancy is an adaptive trait whereby germination is blocked under favourable conditions to avoid germination out of season. Over time mature dry seeds lose dormancy and gradually acquire the capacity to germinate. Dormancy levels, i.e. the time required to release dormancy of the newly produced dry seed, are influenced by the environment of the mother plant and particularly by cold temperatures, which increase dormancy levels. It is also known that the seed coat, a maternal dead tissue, is important to keep seeds dormant over time, probably by shielding the seed living tissues from atmospheric oxygen. Biochemical and genetical evidence previously established that cold promotes polyester accumulation in the seed coat and mutant seeds deficient in polyester biosynthesis have low dormancy and viability. However, it is unclear which seed coat structures, such as apoplastic barriers, are remodeled or created de novo in response to cold during seed development. Combining histological and genetical approaches, a previously uncharacterized apoplastic barrier located in the outer integument 1 (oi1) cell layer was discovered. This barrier is strongly reinforced by cold and this process requires a MYB107 transcription factor , specifically expressed in oi1 cells. myb107 mutants is lacking the MYB107 TF, lacking the barrier and are less dormant, thus providing direct evidence that this barrier promotes dormancy in Arabidopsis.

2025-01-27 | GSE250191 | GEO

Project description:Studies of Siberia lily dormancy release process

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Analysis of light induced secondary seed dormancy in Aethionema arabicum, Cyprus accession

Project description:Light inhibits the seed germination in the Cypriot accession (CYP) of Aethionema arabicum. Extended light illumination also induces a secondary seed dormancy that inhibits the germination even if the seeds were transferred back to darkness. This analysis aims to compare the seed transcriptome before or after the dormancy was established by light illumination (100 µmol/m2s; white light). One day light treatment (1dL) inhibits the germination but the seeds are not yet dormant. Seven day (7dL) or 14-day (14dL) long light treatment induces dormancy, and the seeds stay dormant after 7 dal-light plus 7 day-dark (7dL7dD) treatment. To understand the dormancy establishment, transcriptome was compared between non-dormant (1dL) and dormant (7dL, 14dL, 7dL7dD) seed samples.

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