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Brycinus nurse

ABSTRACT: Brycinus nurse

PROVIDER: PRJNA1081329 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR28200092_1.fastq.gz	Fastqsanger.gz
	SRR28200092_2.fastq.gz	Fastqsanger.gz

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Nurse cell-derived small RNAs define paternal epigenetic inheritance in Arabidopsis

Project description:We report here that gentic methylation in the male germline, from meiocytes to sperm, is established by siRNAs transcribed from transposons with imperfect sequence homology. These siRNAs are synthesized by meiocyte nurse cells (tapetum) via activity of the chromatin remodeler CLASSY3, which is specifically expressed in tapetal cells. Plants that produce siRNAs only in the tapetum have broadly normal DNA methylation of the entire germline. Finally, we also report that these nurse cell-derived siRNAs (niRNAs) silence germline transposons, thereby safeguarding genome integrity. Our results reveal the crucial role of tapetal niRNAs in germline methylation reprogramming, which is remarkably analogous to piRNA-mediated reprogramming in animal germlines.

2021-07-01 | GSE161625 | GEO

Regulation of Brain Gene Expression in Apis melifera Nurse and Forager

Project description:The aim of this work was to extend the previous gene expression analysis ofM- M- nurse and forager gene expression, but using the Apis oligo-array designed from the recently sequenced genome.M- M- This study included 28000 features which included ~11000 predicted genes.M- M- The intial study was done using a microarray design based on PCR products of 5500 features derived from an expressed sequence tag analysis of a brain cDNA library.M- M- The variables studied were age (young and old), cast (nurse and forager) and differential gene expression.

2011-03-09 | E-TABM-658 | biostudies-arrayexpress

Comparison of the translatome of follicle cells during developmental phagoptosis and starvation-induced apoptotic death of nurse cells in Drosophila melanogaster oogenesis.

Project description:The death and clearance of nurse cells is a consequential milestone in Drosophila melanogaster oogenesis. In preparation for oviposition, the germline-derived nurse cells bequeath to the developing oocyte all their cytoplasmic contents and undergo programmed cell death. The death of the nurse cells is controlled non-autonomously and is precipitated by epithelial follicle cells of somatic origin acquiring a squamous morphology and acidifying the nurse cells externally. Alternatively, stressors such as starvation can induce the death of nurse cells earlier in mid-oogenesis, manifesting apoptosis signatures, followed by their engulfment by epithelial follicle cells. To identify and contrast the molecular pathways underlying these morphologically and genetically distinct cell death paradigms, both mediated by follicle cells, we compared their genome-wide translational profiles before and after differentiating to acquire a phagocytic capability, as well as during well-fed and nutrient-deprived conditions. By coupling the GAL4-UAS system to Translating Ribosome Affinity Purification (TRAP-seq) we performed high-throughput screens to identify pathways selectively activated or repressed by follicle cells to employ nurse cell-clearance routines contextually and preferentially. Our analyses and in vivo follow-up studies identified several key genes and pathways that play vital roles during the making of a healthy egg, such as maintaining the oocyte reserve, ensuring structural integrity of the egg chamber, and regulating metabolic and signaling pathways.

2024-12-10 | GSE276035 | GEO

Epigenome analysis of monocytes and derived nurse-like cells from chronic lymphocytic leukemia patients, along with healthy-donor controls

Project description:Monocytes and matching culture-derived nurse-like cells were compared using Illumina's EPIC/850k arrays Monocytes isolated using MACS and NLCs isolated using cytometry sorting as CD14+/CD19-

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Gene expression profile of Nurse-Like Cells (NLC) derived from chronic lymphocytic leukemia

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2022-08-12 | PXD032158 | Pride

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Functional type of nurse plants determines primary soil bacterial succession

Project description:Functional type of nurse plants determines primary soil bacterial succession

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Genome Assembly of short-tail nurse shark (Pseudoginglymostoma brevicaudatum) - ORG.one

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Gene expression profile of Nurse-Like Cells (NLC) derived from chronic lymphocytic leukemia

2012-12-11 | E-GEOD-42733 | biostudies-arrayexpress