Project description:Streptomyces sp. M7 has demonstrated ability to remove lindane from culture media and soils. In this study, we used MS-based label-free quantitative proteomic to understand lindane degradation and its metabolic context in Streptomyces sp. M7. We identified the proteins involved in the up-stream degradation pathway. Our results demonstrated that mineralization of lindane is feasible since proteins from an unusual down-stream degradation pathway were also identified. Degradative steps were supported by an active catabolism that supplied energy and reducing equivalents in the form of NADPH. This is the first study in which degradation steps of an organochlorine compound and metabolic context are elucidate in a biotechnological genus as Streptomyces. These results serve as basement to study other degradative actinobacteria and to improve the degradation processes of Streptomyces sp. M7.
Project description:Competition between bacteria can impact the ability to colonize a host. In the accessory nidamental gland (ANG) reproductive symbiosis of the Hawaiian bobtail squid, Euprymna scolopes, symbionts colonize various epithelium lined tubules of adult females. We hypothesize that symbionts may use competitive mechanisms to determine ANG tubule colonization outcomes. One ANG strain, Leisingera sp. ANG-M7, was found to inhibit another ANG symbiont, Leisingera sp. ANG-DT, in vitro. Transposon mutagenesis was used to determine the genes required for this antimicrobial activity. Seven ANG-M7 transposon mutants were found with significantly decreased ability to inhibit ANG-DT compared to WT ANG-M7. Of these mutants, three strains (P08F1, P24E3, P40B12) were selected for transcriptome sequencing to look for shared pathways that were significantly different from WT.
