Project description:Increased heavy rainfall cause important drops in salinity to values close to 0, which can persist for several days in estuaries. Lethal and sublethal physiological and behavioural effects of decreases in salinity below ten have already been found to occur in the commercially relevant clam species Venerupis corrugata, Ruditapes decussatus and R. philippinarum and the cockle Cerastoderma edule, which generate ~74 million euros annually in Galicia (NW Spain). However, studies of the molecular response to hyposaline stress in bivalves are scarce. This ‘shotgun’ proteomics study evaluates changes in mantle-edge proteins subjected to short-term hyposaline episodes in two different months (March and May) during the gametogenic cycle. We found evidence that the effects of the gametogenic cycle on the proteome are greater than those related to the salinity treatments. However, hyposalinity modulated proteome profiles for both months in V. corrugata and C. edule and on R. philippinarum in May, involving proteins implicated in ROS production, redox homeostasis, cytoskeleton modulation and the activation of apoptotic, autophagic and lipid degradation pathways. Nevertheless, essential proteins for an optimal osmotic stress response but high energy demandants, such as chaperones, osmoprotectants and DNA repair factors, were in both large and small abundance under hyposalinity for those species. In both time points for R. decussatus and R. philippinarum in March, almost no differences between treatments were detected. These exploratory results reinforce the interest in the specific study and conservation of native species C. edule and V. corrugata, particularly sensitive to hyposalinity.
Project description:The Manila clam (Ruditapes philippinarum) is a cultured bivalve species with high worldwide commercial importance. Nevertheless, diseases can cause high economical losses. For this reason, the study of immune genes in bivalve mollusks has increased in the last years. The present work describes the construction of the first R. philippinarum microarray containing immune-related hemocyte sequences and its application for the study of the gene transcription profiles of hemocytes from clams challenged with Vibrio alginolyticus through a time course.
Project description:This SuperSeries is composed of the following subset Series: GSE22915: Mussel (Mytilus galloprovincialis) digestive gland tissue: gene expression profiles across an annual cycle GSE23049: Mytilus galloprovincialis: development of female gonads GSE23050: Mytilus galloprovincialis: development of male gonads GSE23051: Mytilus galloprovincialis: differences between male and female gene expression patterns in gonads (mantle tissue) Refer to individual Series
Project description:Four pools of digestive glands were treated as biological replicates in order to evaluate the repeatability of Ruditapes philippinarum oligo microarray platform.
Project description:A Ruditapes philippinarum microarray platform was developed to identify digestive gland gene expression profiles in response to 100 µg /l and 1000 µg/l ibuprofen exposure.
