Project description:We conducted 16S rRNA sequencing analyse on colonic contents to evaluate whether forced loss led to alterations in gut microbiota composition and function.
Project description:<p>In mice induced by DSS, oral administration of silibinin was used for intervention, and then the colonic contents were removed for LC-MS non-targeted metabolomics analysis. In mice induced by DSS, colonic contents were taken, fecal bacteria were extracted, and co-cultured with silibinin ex vivo. Subsequently, metabolomics analysis was performed using the same method.</p>
Project description:his study investigated the metabolic alterations in colonic contents associated with aqueous extract of Artemisia argyi (AEAA) intervention in a lipopolysaccharide (LPS)-induced acute lung injury (ALI) mouse model. Male C57BL/6 mice were randomly divided into control, LPS, and AEAA treatment groups with low-, medium-, and high-dose administration.</p><p>AEAA was administered by oral gavage for 28 consecutive days. On day 29, ALI was induced by intratracheal instillation of LPS (5 mg/kg) in the LPS and treatment groups, while control mice received sterile saline. Colonic content samples were collected 8 hours after LPS administration.</p><p>Untargeted metabolomics analysis was performed using liquid chromatography¨Cmass spectrometry (LC-MS). Quality control (QC) samples were included to ensure analytical stability. The metabolomic profiles of colonic contents were analyzed to explore gut metabolic alterations and potential mechanisms underlying the protective effects of AEAA in acute lung injury.</p><p>This dataset provides raw LC-MS data of colonic content metabolomics and can be used to investigate gut-related metabolic pathways involved in inflammation and natural product intervention.
Project description:We investigated transcriptomic changes in mouse organoids treated with colonic contents from diet-induced obese and normal-weight rats. 2D Mouse organoids were harvested after 40 h of treatment with 10% solutions of rat colonic content. Our findings revealed induction of local inflammatory processes under treatment with content derived from rats fed a high-fat diet.
