Project description:4 cancer cell lines were treated with T-025 for 6 hours T-025 is a novel small molecule inhibitor of CLK. CLK is a kinase that regulates pre-mRNA splicing.
Project description:In order to identify interacting proteins of Pdcd7 (U11-59K), pCMV6-Pdcd7-Myc was transfected in HEK293T cells, followed by UV cross-linking and immunoprecipitation with Myc antibody. Samples were then analysed on Thermo Scientific Q Exactive HF mass spectrometer.