Project description:Macrophage-mediated inflammation is a key pathological feature of the injured tendon-to-bone interface after rotator cuff tear. In this study, we performed bulk RNA sequencing to investigate the transcriptional effects of low-intensity ultrasound on inflammatory macrophage activation. RAW264.7 macrophages were stimulated with lipopolysaccharide to establish an inflammatory macrophage model and were treated with ultrasound at 50 mW/cm² under fixed low-intensity pulsed ultrasound settings. RNA-seq was performed to compare LPS-stimulated macrophages with or without 50 mW/cm² ultrasound treatment. Transcriptomic analysis showed that 50 mW/cm² ultrasound substantially reprogrammed the inflammatory transcriptional state of LPS-stimulated RAW264.7 macrophages. Multiple inflammation-associated genes, including Il1a, Il12b, Tnf, Il6, Ccl7 and Ccl4, were downregulated after ultrasound treatment. Gene Ontology enrichment analysis indicated suppression of inflammation-related biological processes, including immune system process, immune response, response to lipopolysaccharide and regulation of inflammatory response. Gene set enrichment analysis further revealed negative enrichment of the TNF signaling pathway and NF-κB signaling pathway. These data provide transcriptomic evidence that 50 mW/cm² ultrasound suppresses macrophage inflammatory activation through inhibition of canonical inflammatory signaling pathways.
Project description:The purpose of this study was to evaluate gene expression differences in debrided human venous ulcer tissue from patients treated with low-frequency (20 kHz), low-intensity (100 mW/cm2) ultrasound compared to a sham treatment in an effort to better understand the potential biological mechanisms.
Project description:Analysis of human peritumoral brain following a low-intensity pulsed ultrasound (LIPU) mediated opening of the blood brain barrier, to enhance the delivery of systemic chemotherapies (paclitaxel or carboplatin).
Project description:Here, the effects of non-thermal low intensity pulsed ultrasound (LIPUS) on the gene expression in human lymphoma U937 cells were investigated using by an Affymetrix GeneChip system. Six hours after LIPUS treatment (0.3 W/cm2 for 1 min), apoptosis (14±3.8%, mean±SD) with minimal cell lysis was observed. At 3 h post-treatment, LIPUS down-regulated 193 genes and up-regulated 201 genes by >1.5-fold. Experiment Overall Design: U937 cells, a human lymphoma cell line, were treated with low intensity pulsed ultrasound (0.3 W/cm2 for 1 min) and followed by incubation for 3 h at 37°C. Non-treated cells were served as control. Total RNA samples were prepared from the cells. Gene expression was analyzed by an Affymetrix GeneChip® system with Human Expression Array U133A which was spotted with 22,283 probe sets. Sample preparation for array hybridization was carried out as described in the manufactureâs instructions.
Project description:Low-intensity pulsed ultrasound (LIPUS) has been applied as a therapeutic adjunct to promote fracture healing. However, the detailed molecular mechanisms by which LIPUS promotes bone fracture healing have not yet been fully elucidated. In the present study, the early response genes elicited by low-intensity pulsed ultrasound (LIPUS) in bone marrow stromal cells (BMSCs) were investigated using GeneChip® oligonucleotide microarrays.
