Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Skatole attenuates osteoarthritis by protecting chondrocytes and mediating macrophage repolarization through regulating the NFκB/MAPK signaling pathway and metabolic reprogramming

ABSTRACT: Skatole attenuates osteoarthritis by protecting chondrocytes and mediating macrophage repolarization through regulating the NFκB/MAPK signaling pathway and metabolic reprogramming

PROVIDER: PRJNA1086869 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR28312012_1.fastq.gz	Fastqsanger.gz
	SRR28312012_2.fastq.gz	Fastqsanger.gz
	SRR28312013_1.fastq.gz	Fastqsanger.gz
	SRR28312013_2.fastq.gz	Fastqsanger.gz
	SRR28312014_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 18

Similar Datasets

Skatole attenuates osteoarthritis by protecting chondrocytes and mediating macrophage repolarization through regulating the NFκB/MAPK signaling pathway and metabolic reprogramming II

Project description:Skatole attenuates osteoarthritis by protecting chondrocytes and mediating macrophage repolarization through regulating the NFκB/MAPK signaling pathway and metabolic reprogramming II

| PRJNA1195129 | ENA

Skatole attenuates osteoarthritis by protecting chondrocytes and mediating macrophage repolarization through regulating the NFκB/MAPK signaling pathway and metabolic reprogramming

Project description:Osteoarthritis (OA) is the most common joint disease, but there are currently no disease-modifying OA drugs (DMOADs) yet approved by the regulatory agencies. Regulating macrophage polarization can alleviate synovial inflammation and then repair articular cartilage damage, providing a new target for OA treatment. Here, we found that Skatole can modulate macrophage polarization and attenuate OA. Skatole hindered M1 macrophage polarization while promoting M2 macrophage polarization. Mechanistically, Skatole activated Stat6, suppressed the phosphorylation of IKK, IκBα, and p65 in NFκB signaling pathway, and inhibited MAPK signaling activation. RNA-seq analysis revealed that Skatole downregulated the expression of inflammation-related genes, while upregulating genes involved in glutathione metabolism and oxidative phosphorylation, thereby reducing ROS levels and inhibiting M1 macrophage polarization. Moreover, conditional medium (CM) from M1 macrophages treated with Skatole balanced anabolism and catabolism in mouse chondrocytes while inhibiting cell apoptosis. Intriguingly, in IL1β-treated chondrocytes, Skatole directly suppressed inflammation and catabolism, without significantly affecting cell apoptosis or anabolism; In vivo experiments showed that Skatole increased M2 polarization and decreased M1 polarization of synovial macrophages, alleviated synovitis, and lessend articular cartilage damage in destabilization of medial meniscus (DMM)-induced OA mice. Our finding suggest that Skatole has the potential to function as a DMOAD.

2025-02-12 | GSE261400 | GEO

Skatole attenuates osteoarthritis by protecting chondrocytes and mediating macrophage repolarization through regulating the NFκB/MAPK signaling pathway and metabolic reprogramming II

2025-02-12 | GSE283748 | GEO

Epigenetic mechanisms mediating cell state transitions in chondrocytes.

Project description:Epigenetic mechanisms mediating cell state transitions in chondrocytes.

| PRJNA649651 | ENA

Epigenetic mechanisms mediating the transition from proliferating into hypertrophic chondrocytes. [ChIP-Seq]

Project description:Epigenetic mechanisms mediating the transition from proliferating into hypertrophic chondrocytes. [ChIP-Seq]

| PRJNA649653 | ENA

Epigenetic mechanisms mediating the transition from proliferating into hypertrophic chondrocytes. [RNA-Seq]

Project description:Epigenetic mechanisms mediating the transition from proliferating into hypertrophic chondrocytes. [RNA-Seq]

| PRJNA649654 | ENA

Epigenetic mechanisms mediating the transition from proliferating into hypertrophic chondrocytes. [RNA-Seq]

Project description:Correlation of RNA-seq and ChIP-seq data from proliferating (PC) and hypertrophic chondrocytes (HC).

2021-03-10 | GSE155400 | GEO

Epigenetic mechanisms mediating the transition from proliferating into hypertrophic chondrocytes. [ChIP-Seq]

Project description:Chromatin Immunopreciptiation of histone modifications in primary proliferating (PC) and hypertrophic chondrocytes (HC) from embryonic mice

2021-03-10 | GSE155399 | GEO

Dynamic magneto-mechanical force in lysosomes instructs durable macrophage repolarization for antitumor immunity.

Project description:Mechanical forces are the emerging physical cue to regulate biochemical signals of immune cells for antitumor immunity. Here, we develop a magneto-mechanical force-triggered lysosomal membrane permeabilization (MagLMP) strategy to dictate durable macrophage repolarization, which leads to dynamic damage of lysosomal membrane via the finely tuned torque-induced vortex. We found that the MagLMP could activate M1-like macrophage repolarization, followed by the mounting of antitumor immunity. Together, we establish an intracellular mechanical strategy to dynamically manipulate innate immune responses in vivo and provide the tool for durable immunotherapy through organelle mechanotransduction.

2025-08-01 | GSE273977 | GEO

LMD-RNAseq of resting chondrocytes and proliferative chondrocytes

Project description:LMD-RNAseq of resting chondrocytes and proliferative chondrocytes

| PRJNA793434 | ENA