Saccharomyces cerevisiae
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PROVIDER: PRJNA108905 | ENA |
REPOSITORIES: ENA
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Project description:Variations in stress sensitivity and genomic expression in diverse S. cerevisiae strains (CGH)
| PRJNA108903 | ENA
Project description:This SuperSeries is composed of the following subset Series: GSE10267: Variations in stress sensitivity and genomic expression in diverse S. cerevisiae strains (CGH) GSE10268: Variations in stress sensitivity and genomic expression in diverse S. cerevisiae strains (gene expression) Keywords: SuperSeries Refer to individual Series
2008-09-05 | E-GEOD-10269 | biostudies-arrayexpress
Project description:We study the genetics, including microarray karyotyping using comparative genomic hybridization, to explore global changes in the genomic DNA of seven S. cerevisiae strains related to traditional fermentations of very different sources comparing to the sequenced S. cerevisiae laboratory strain (S288C). Our final goal is to determine the adaptive evolution of properties of biotechnological interest in Saccharomyces yeasts. Many copy number variations (CNVs) were observed, especially in genes associated to subtelomeric regions and transposon elements. Among the fermentation strains, differential CNV was observed in genes related to sugar transport and metabolism. An outstanding example of diverse CNV is the gen PUT1, involved in proline assimilation, which correlated with the adaptation of the strains to the presence of this nitrogen source in the media.
2013-12-15 | GSE46165 | GEO
Project description:We developed an artificial genome evolution system, which we termed ‘TAQing’, by introducing multiple genomic DNA double-strand breaks using a heat-activatable endonuclease in mitotic yeast. The heat-activated endonuclease, TaqI, induced random DSBs, which resulted in diverse types of chromosomal rearrangements including translocations. Array comparative genomic hybridization (aCGH) analysis was performed with cell-fused Saccharomyces cerevisiae strains induced genome evolution by TAQing system. Some of copy number variations (CNVs) induced by massive genome rearrangements were detected in the TAQed yeast strains.
2018-05-02 | GSE90025 | GEO
Project description:We study the genetics, including microarray karyotyping using comparative genomic hybridization to explore global changes in the genomic DNA, of four S. bayanus var uvarum strains related to traditional fermentations of very different sources comparing to the sequenced S. cerevisiae laboratory strain (S288C). Our final goal is to determine the adaptive evolution of properties of biotechnological interest in Saccharomyces yeasts. Many copy number variations (CNV) were observed, especially in genes associated to subtelomeric regions and transposon elements. Among the fermentation strains, differential CNV was observed in genes related to sugar transport and metabolism. An outstanding example of diverse CNV is the gen PUT1, involved in proline assimilation, which correlated with the adaptation of the strains to the presence of this nitrogen source in the media.
2016-04-15 | GSE67917 | GEO
Project description:We study the genetics, including microarray karyotyping using comparative genomic hybridization, to explore global changes in the genomic DNA of seven S. cerevisiae strains related to traditional fermentations of very different sources comparing to the sequenced S. cerevisiae laboratory strain (S288C). Our final goal is to determine the adaptive evolution of properties of biotechnological interest in Saccharomyces yeasts. Many copy number variations (CNVs) were observed, especially in genes associated to subtelomeric regions and transposon elements. Among the fermentation strains, differential CNV was observed in genes related to sugar transport and metabolism. An outstanding example of diverse CNV is the gen PUT1, involved in proline assimilation, which correlated with the adaptation of the strains to the presence of this nitrogen source in the media. Seven S. cerevisiae strains were obtained from natural environments and different fermentation processes. The S. cerevisiae strain S288C was used as a control for microarray hybridizations. All experiments were performed using duplicate arrays, and Cy5-dCTP and Cy3-dCTP dye-swap assays were performed to reduce dye-specific bias.
2013-12-15 | E-GEOD-46165 | biostudies-arrayexpress
Project description:Comparative Genomic Hybridization of natural Saccharomyces cerevisiae strains vs lab reference strains.
2020-12-31 | GSE136466 | GEO
Project description:Timely signaling pathways activation allows cells to survive diverse environmental stress conditions. Mitogen-activated protein kinases (MAPKs) are a highly conserved class of signaling molecules in eukaryotes with essential functions in cellular responses to stress. In Saccharomyces cerevisiae, the role of MAPK Hog1 as a master regulator of the coordinated response to osmotic stress is well understood. However, recent findings suggest that the role of Hog1 may extend beyond canonical osmoadaptation. This study investigates the role of Hog1 in mediating transcriptional responses to acute oxidative and ethanol stress. By harnessing the natural variation present in wild strains of S. cerevisiae, we use gene knockouts, comparative transcriptomics, and survival assays to determine Hog1’s involvement in stress responses beyond osmoadaptation. Our findings demonstrate that Hog1 mediates transcriptional reprogramming for non-osmotic stress response in a strain-dependent manner. Osmospecificity of Hog1 activity was identified in the DBY8268 laboratory strain, while differential gene expression was observed in HOG1 knockouts of all wild strains tested under both oxidative and ethanol stress. Further, our data indicate that the function of Hog1 in the response to non-osmotic stress is distinct from the canonical response, with effects ranging from altered ribosomal protein expression dynamics to altered environmental stress response (ESR) activity. Differences in expression correlate with fitness defects of hog1∆ mutants. These results suggest a generalized role of the Hog1 MAPK in S. cerevisiae, consistent with an evolutionarily generalized function for this kinase, underscoring the importance of genomic diversity for elucidating stress signalling dynamics in yeast.
2026-01-29 | GSE312455 | GEO
Project description:We expressed human RECQL5 in the model organism Sacharomyces cerevisiae. We found that RECQL5 overexpression in yeast leads to cell growth inhibition and increased genotoxic sensitivity. We carried-out transcriptome analysis of yeast strains overexpressing human RECQL5 helicase to explore its impact on gene expression.
2025-01-15 | GSE180112 | GEO