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Identification of Chromatin proteins involved in gene repression in lamina-associated domains (pA-DamID)

ABSTRACT: Identification of Chromatin proteins involved in gene repression in lamina-associated domains (pA-DamID)

PROVIDER: PRJNA1089230 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28380444.fastq.gz	Fastqsanger.gz
	SRR28380445.fastq.gz	Fastqsanger.gz
	SRR28380446.fastq.gz	Fastqsanger.gz
	SRR28380447.fastq.gz	Fastqsanger.gz
	SRR29714276.fastq.gz	Fastqsanger.gz

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Similar Datasets

Identification of Chromatin proteins involved in gene repression in lamina-associated domains (pA-DamID)

Project description:Lamina-associated domains (LADs) are large chromatin regions that are associated with the nuclear lamina and form a repressive environment for transcription. The molecular players that mediate gene repression in LADs are currently unknown. Here we performed a full-genome genetic screen in human cells using LAD-integrated fluorescent reporters to identify such regulators. Surprisingly, the screen identified very few lamina proteins, but revealed roles for dozens of known chromatin regulators. Among these are the negative elongation factor (NELF) complex and interacting factors, suggesting that regulation of RNA polymerase pausing can be a mechanism to repress transcription in LADs. Furthermore, the chromatin remodeler complex BAF and the activation complex Mediator can work both as activators and repressors in LADs, depending on the local context and possibly rewiring of heterochromatin. Our data suggest that fundamental regulatory steps of the transcription process and chromatin remodeling, rather than interaction with NL proteins, have a major role in the regulation of transcription in LADs.

2024-07-10 | GSE261868 | GEO

Genome-wide maps of nuclear lamina interactions in AML12 cells upon MATR3 KD [DamID-Seq]

Project description:We performed DamID-seq assay in WT and MATR3-depleted AML12 cells to investigate the Lamina-associated-domains (LADs) affected by loss of MATR3.

2025-03-14 | GSE200242 | GEO

DamID LaminB1 data in mouse MEFs, wild-type and POU2F1-/-. DamID LaminA data in mouse Neural Precursor Cells and Astrocytes.

Project description:DamID LaminB1 data were generated in POU2F1-/- MEFs to study the potential role of POU2F1/Oct1 in genome - nuclear lamina interactions. DamID LaminA data were generated in NPCs and Astrocytes to study similarities/differences between LaminA and LaminB1 binding. Comparison of MEF wt versus MEF POU2F1-/-. Comparison of LaminA (NPC & AC) with LaminB1 (NPC & AC data in GSE17051)

2012-10-10 | E-GEOD-36132 | biostudies-arrayexpress

Interactions between the genome and the nuclear lamina are multivalent and cooperative (pA-DamID)

Project description:Interactions between the genome and the nuclear lamina are multivalent and cooperative (pA-DamID)

| PRJNA1053340 | ENA

Effects of transcription on genome - nuclear lamina interactions: DamID-seq data

Project description:In mammalian nuclei, transcriptionally active genomic regions tend to localize to the interior of the nucleus while inactive regions are often located at at the nuclear lamina. In this study we activated specific genes in lamina-associated domains by TALE-VP64 or CRISPRa-mediated activation. We also reduced transcription of individual genes by knockout of promoter/enhancer regions, or by insertion of a transcription termination sequence. In each case we generated genome-wide DamID maps to determine changes in nuclear lamina interactions, and in selected cases we also generated Repli-seq maps and/or RNAseq data.

2019-06-28 | GSE133272 | GEO

Identification of Chromatin proteins involved in gene repression in lamina-associated domains (RNA-Seq)

2024-07-10 | GSE261953 | GEO

DNA Adenine Methyltransferase Identification (DamID) Coupled to Next Generation Sequencing For Profiling Lamina Associated Domains

Project description:DNA Adenine Methyltransferase Identification (DamID) Coupled to Next Generation Sequencing For Profiling Lamina Associated Domains

| PRJNA526868 | ENA

Identification of Chromatin proteins involved in gene repression in lamina-associated domains

Project description:Identification of Chromatin proteins involved in gene repression in lamina-associated domains

| PRJNA1089617 | ENA

Coordinated control of genome-nuclear lamina interactions by Topoisomerase 2B and Lamin B receptor (pA-DamID)

Project description:Coordinated control of genome-nuclear lamina interactions by Topoisomerase 2B and Lamin B receptor (pA-DamID)

| PRJNA1162536 | ENA

LaminB1 DamID analysis in RIF1 depleted preimplantation mouse embryos

Project description:We investigated how RIF1 depletion affects the nuclear organization in mouse preimplantation embryos by performing DamID-seq to map lamina-associated domains (LADs).

2025-03-18 | GSE262788 | GEO

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