Pilea menghaiensis
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ABSTRACT:
PROVIDER: PRJNA1090229 | ENA |
REPOSITORIES: ENA
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Project description:BackgroundPilea is a genus of perennial herbs from the family Urticaceae, and some species are used as courtyard ornamentals or for medicinal purposes. At present, there is no information about the plastid genome of Pilea, which limits our understanding of this genus. Here, we report 4 plastid genomes of Pilea taxa (Pilea mollis, Pilea glauca 'Greizy', Pilea peperomioides and Pilea serpyllacea 'Globosa') and performed comprehensive comparative analysis.ResultsThe four plastid genomes all have a typical quartile structure. The lengths of the plastid genomes ranged from 150,398 bp to 152,327 bp, and each genome contained 113 unique genes, including 79 protein-coding genes, 4 rRNA genes, and 30 tRNA genes. Comparative analysis showed a rather high level of sequence divergence in the four genomes. Moreover, eight hypervariable regions were identified (petN-psbM, psbZ-trnG-GCC, trnT-UGU-trnL-UAA, accD-psbI, ndhF-rpl32, rpl32-trnL-UAG, ndhA-intron and ycf1), which are proposed for use as DNA barcode regions. Phylogenetic relationships based on the plastid genomes of 23 species of 14 genera of Urticaceae resulted in the placement of Pilea in the middle and lower part of the phylogenetic tree, with 100% bootstrap support within Urticaceae.ConclusionOur results enrich the resources concerning plastid genomes. Comparative plastome analysis provides insight into the interspecific diversity of the plastid genome of Pilea. The identified hypervariable regions could be used for developing molecular markers applicable in various research areas.
| S-EPMC7792329 | biostudies-literature
Project description:Primary objectives: The primary objective is to investigate circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
Primary endpoints: circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
| 2533916 | ecrin-mdr-crc
Project description:Genome-wide transcriptome sequencing at different stages of Drosophila melanogaster development, RNA-seq
2020-07-22 | E-GEOD-18068 | ExpressionAtlas
Project description:Three hitherto undescribed species of Pilea (Urticaceae) from limestone karst in China are described and illustrated. Affinities of the species are discussed and Global Species Conservation Assessments presented. The new species are Pilea cavernicola A.K. Monro, C.J. Chen & Y.G. Wei, sp. nov. (Vulnerable) which most closely resembles Pilea scripta (Buch.-Ham. ex D.Don) Wedd. and Pilea gracilis Handel-Mazzetti, Pilea shizongensis A.K. Monro, C.J. Chen & Y.G. Wei, sp. nov. (Endangered) which is most similar to Pilea aquarum Dunn and Pilea guizhouensis A.K. Monro, C.J. Chen & Y.G. Wei, sp. nov. (Vulnerable) which resembles Pilea boniana Gagnep. and Pilea rubriflora C. Wright mostclosely.
| S-EPMC3597003 | biostudies-literature