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Targeting GI-SINE (Growth inducing B2-SINE) RNA in DRG neurons by Antisense Oligonucleotides (ASO)

ABSTRACT: Targeting GI-SINE (Growth inducing B2-SINE) RNA in DRG neurons by Antisense Oligonucleotides (ASO)

PROVIDER: PRJNA1090485 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR28411573_1.fastq.gz	Fastqsanger.gz
	SRR28411573_2.fastq.gz	Fastqsanger.gz
	SRR28411574_1.fastq.gz	Fastqsanger.gz
	SRR28411574_2.fastq.gz	Fastqsanger.gz
	SRR28411575_1.fastq.gz	Fastqsanger.gz

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Targeting GI-SINE (Growth inducing B2-SINE) RNA in DRG neurons by Antisense Oligonucleotides (ASO)

Project description:We identified 2 specific motifs that are enriched in GI-SINE RNA expressed in sciatic-nerve injured DRG neurons compared to non-regulated B2-SINE RNAs. We designed a mix of 5 ASOs (21nt phosphorothioate DNA with LNA-flanks) that target these motifs and transfected those into cultured dorsal root ganglia (DRG) neurons. Control ASO was based on a 21nt non-targetting sequence from shControl backbone (addgene plasmid #85741). Primary DRG neurons were transfected using Dharmafect-4 with ASO at 50nM final concentration 1 hour after plating. Cultures were processed for RNA extraction 48h after transfection for RNA sequencing to identify changes in B2-SINE expression.

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